Chan Sze Mun, Khoo Kong Soo, Sekaran Shamala Devi, Sit Nam Weng
Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia.
Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Bandar Barat, Kampar 31900, Malaysia.
Plants (Basel). 2021 Aug 11;10(8):1658. doi: 10.3390/plants10081658.
The lack of specific treatment for chikungunya fever makes the need for anti-chikungunya virus agents more crucial. This study was conducted to evaluate 132 extracts obtained by sequential solvent extraction from 21 medicinal plants for cytopathic effect inhibitory activity using virus-infected Vero cells in two different sample introduction modes. Among the extracts, 42 extracts (31.8%) from 12 plants in the concurrent mode and three extracts (2.3%) from a plant in the non-concurrent mode displayed strong cytopathic effect inhibitory activity (cell viability ≥70%). Viral load quantification analysis unveiled that the extracts of (chloroform, ethyl acetate, and ethanol), (ethanol), and (ethanol and methanol) hindered the release of viral progeny from the infected cells while the extracts of (ethanol), (water), (water), and (chloroform and ethyl acetate) blocked the entry of virus into the cells. The extracts of (ethyl acetate), (chloroform, ethyl acetate, and ethanol), and (ethanol) possessed virucidal effect and caused 5.41-log to 6.63-log reductions of viral load compared to the virus control. The results indicate that these medicinal plants are potential sources of anti-chikungunya virus agents that have varied modes of action.
由于缺乏针对基孔肯雅热的特异性治疗方法，对抗基孔肯雅病毒药物的需求变得更加迫切。本研究旨在评估通过连续溶剂萃取从21种药用植物中获得的132种提取物，采用两种不同的样品引入模式，利用病毒感染的Vero细胞评估其对细胞病变效应的抑制活性。在这些提取物中，12种植物的42种提取物（31.8%）在同时模式下以及1种植物的3种提取物（2.3%）在非同时模式下表现出较强的细胞病变效应抑制活性（细胞活力≥70%）。病毒载量定量分析表明，[植物名称1]（氯仿、乙酸乙酯和乙醇提取物）、[植物名称2]（乙醇提取物）以及[植物名称3]（乙醇和甲醇提取物）阻碍了病毒后代从感染细胞中的释放，而[植物名称4]（乙醇提取物）、[植物名称5]（水提取物）、[植物名称6]（水提取物）以及[植物名称7]（氯仿和乙酸乙酯提取物）则阻止了病毒进入细胞。[植物名称8]（乙酸乙酯提取物）、[植物名称9]（氯仿、乙酸乙酯和乙醇提取物）以及[植物名称10]（乙醇提取物）具有杀病毒作用，与病毒对照相比，病毒载量降低了5.41个对数至6.63个对数。结果表明，这些药用植物是具有多种作用方式的抗基孔肯雅病毒药物的潜在来源。
