Institute of Materials Research and Engineering, A*STAR Singapore, Singapore.
Department of Chemistry, National Taiwan University, Taipei, Taiwan.
Biosens Bioelectron. 2017 Oct 15;96:167-172. doi: 10.1016/j.bios.2017.05.005. Epub 2017 May 4.
The binding of organometallic osmium carbonyl clusters onto the surface of gold nanoparticles (CO-Au NPs) greatly enhanced the CO stretching vibration signal at ~2100cm, which is relatively free from interference due to the absorbance of biomolecules. By utilizing the acetylcholinesterase (AChE) mediated hydrolysis of acetylthiocholine to thiocholine where the activity of AChE is inhibited by the presence of organophosphate pesticides (OPPs), the subsequent thiocholine-induced aggregation of CO-Au NPs can be monitored by the change in color of the NPs solution and the variation in intensity of the SERS CO signal. The change in color offers a fast pre-screening method, whereas monitoring via SERS is used for greater accuracy and lower limit of detection (0.1 ppb) for quantitative detection. Its potential as a quick and accurate method of OPPs monitoring in consumer products was demonstrated in the detection of OPPs in real spiked samples such as beer.
将有机金属锇羰基簇合物结合到金纳米粒子（CO-Au NPs）的表面上，大大增强了在 ~2100cm 处的 CO 伸缩振动信号，该信号相对不受生物分子吸收的干扰。通过利用乙酰胆碱酯酶（AChE）介导的乙酰硫代胆碱的水解生成硫代胆碱，AChE 的活性被有机磷农药（OPPs）的存在所抑制，随后可以通过 NPs 溶液颜色的变化和 SERS CO 信号强度的变化来监测 CO-Au NPs 的聚集。颜色变化提供了一种快速的预筛选方法，而通过 SERS 进行监测则用于更高的准确性和更低的检测限（0.1 ppb）进行定量检测。它在检测实际加标样品（如啤酒）中的 OPPs 时，展示了作为一种快速准确的 OPPs 监测方法的潜力。
