Dogan Mehmet Sinan, Yavas Mehmet Cihan, Yavuz Yasemin, Erdogan Sait, Yener İsmail, Simsek İbrahim, Akkus Zeki, Eratilla Veysel, Tanik Abdulsamet, Akdag Mehmet Zulkuf
Department of Pediatric Dentistry, Faculty of Dentistry, Harran University, Şanlıurfa.
Department of Biophysics, Faculty of Medicine.
Drug Des Devel Ther. 2017 May 4;11:1393-1398. doi: 10.2147/DDDT.S132308. eCollection 2017.
The purpose of this study was to examine the possible effect of extremely low-frequency electromagnetic fields (ELF-EMFs), from a high-voltage source, on rat teeth in terms of changes in trace elements (TEs) and the effect of antioxidants (melatonin [MLT] and [GL]) in counteracting these effects. We used adult male Wistar albino rats with a mean weight of 250-300 g and divided the rats into eight groups. The groups were subjected to an ELF-EMF that was applied with a high-voltage line for 8 hours/day for 26 days (Groups I, II, and III) or 52 days (Groups V, VI, and VII). Groups IV and VIII were the 26- and 52-day control/sham groups, respectively. Groups II and VI were treated with GL, and Groups III and VII were treated with MLT. MLT and GL were administered daily based on the weight of the animals and appropriate standards. At the end of the study, the rats were euthanized, and their anterior teeth were extracted. The teeth were preserved in pure water before evaluating the major TEs. At the end of the study, TE concentrations (in mg/kg) were assessed in the control and test groups. Compared with Group V, statistically significant differences in the concentrations of zinc (Zn) and strontium (Sr) were found for Group VII (ELF-EMF + MLT) (<0.05). Therefore, ELF-EMF exposure can change the content of certain TEs in teeth and, after administering MLT and GL, the values of some of the TEs return to normal.
本研究的目的是从微量元素（TEs）变化方面，研究来自高压源的极低频电磁场（ELF - EMFs）对大鼠牙齿的可能影响，以及抗氧化剂（褪黑素[MLT]和[GL]）在抵消这些影响方面的作用。我们使用平均体重为250 - 300克的成年雄性Wistar白化大鼠，并将大鼠分为八组。这些组接受通过高压线施加的ELF - EMF，每天8小时，持续26天（第一、二和三组）或52天（第五、六和七组）。第四组和第八组分别是26天和52天的对照/假手术组。第二组和第六组用GL处理，第三组和第七组用MLT处理。MLT和GL根据动物体重和适当标准每日给药。在研究结束时，将大鼠安乐死，并拔出它们的前牙。在评估主要TEs之前，将牙齿保存在纯水中。在研究结束时，评估对照组和测试组中的TE浓度（mg/kg）。与第五组相比，发现第七组（ELF - EMF + MLT）的锌（Zn）和锶（Sr）浓度存在统计学显著差异（<0.05）。因此，暴露于ELF - EMF可改变牙齿中某些TEs的含量，在给予MLT和GL后，一些TEs的值恢复正常。
