用于活细胞成像的溶酶体特异性光可激活探针的合成与性质

Tran Mai N, Rarig Robert-André F, Chenoweth David M

Department of Chemistry , University of Pennsylvania , 231 South 34th Street , Philadelphia , PA 19104-6323 , USA . Email:

Department of Chemistry , Temple University , 130 Beury Hall, 1901 N. 13th Street , Philadelphia PA 19122 , USA.

Chem Sci. 2015 Aug 14;6(8):4508-4512. doi: 10.1039/c5sc01601k. Epub 2015 Jun 17.

Fluorescent dyes have become increasingly important in cell biology since they enable high signal-to-noise and selectivity in visualizing subcellular organelles. Photoactivatable dyes allow for tracking and monitoring of a subset of cells or organelles. Here, we report the synthesis and application of a new class of large Stokes shift fluorescent dyes that are water-soluble, cell permeable, non-cytotoxic, and lysosome-specific. Additionally, we demonstrate temporally controlled sequential photoactivation of individual cells in close spatial proximity.

自荧光染料能够在可视化亚细胞器时实现高信噪比和选择性以来，它们在细胞生物学中变得越来越重要。光激活染料可用于追踪和监测细胞或细胞器的一个子集。在此，我们报告了一类新型大斯托克斯位移荧光染料的合成与应用，这类染料具有水溶性、细胞渗透性、无细胞毒性且对溶酶体具有特异性。此外，我们展示了在紧密空间邻近区域对单个细胞进行时间可控的顺序光激活。