一种“可点击”光致变色小分子荧光探针，可用于追踪单个生物分子复合物。
A "Clickable" Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes.
机构信息
Department of Chemistry , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
Department of Pathology and Laboratory Medicine , Center for Neurodegenerative Disease Research, University of Pennsylvania , 3600 Spruce Street , Philadelphia , Pennsylvania 19104 , United States.
出版信息
J Am Chem Soc. 2019 Feb 6;141(5):1893-1897. doi: 10.1021/jacs.8b13094. Epub 2019 Jan 28.
Abstract
Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.
摘要
光转化荧光团可实现对特定生物分子、复合物和细胞区室的可视化和追踪，并具有精确的时空控制。光转化探针领域主要由荧光蛋白变体主导，由于其体积较大，可能会对靶生物分子造成干扰。在这里，我们提出了一种光转化的小分子，称为 CPX，可以通过叠氮-炔环加成（“点击”反应）与任何靶标连接。为了证明其用途，我们将 CPX 应用于研究（1）生物相关合成囊泡的运输和（2）α-突触核蛋白（αS）病理传递涉及的细胞内过程。我们的结果表明，CPX 可以作为一种非侵入性探针，用于追踪生物分子的动力学。
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