Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland.
Departments of Ophthalmology and Orthopaedics & Traumatology, Tampere University Hospital, Tampere, Finland.
Immun Inflamm Dis. 2017 Sep;5(3):280-288. doi: 10.1002/iid3.168. Epub 2017 May 11.
R-Ras GTPase has recently been implicated in the regulation of immune functions, particularly in dendritic cell (DC) maturation, immune synapse formation, and subsequent T cell responses.
Here, we investigated the role of R-Ras in allergen-induced immune response (type 2 immune response) in Rras deficient (R-Ras KO) and wild type (WT) mice.
Initially, we found that the number of conventional DC's in the lymph nodes (LNs) was reduced in R-Ras KO mice. The expression of co-stimulatory CD80 and CD86 molecules on these cells was also reduced on DC's from the R-Ras KO mice. However, there was no difference in papain-induced immune response between the R-Ras WT and KO as measured by serum IgE levels after the immunization. Interestingly, neither the DC number nor co-stimulatory molecule expression was different between WT and R-Ras KO animals after the immunization.
Taken together, despite having reduced number of conventional DC's in the R-Ras KO mice and low expression of CD80 on DC's, the R-Ras KO mice are capable of mounting papain-induced IgE responses comparable to that of the WT mice. To our knowledge, this is the first report addressing potential differences in in vivo allergen responses regulated by the R-Ras GTPase.
Ras 家族 GTP 酶中的 R-Ras 最近被牵涉到免疫功能的调节中，特别是在树突状细胞（DC）成熟、免疫突触形成以及随后的 T 细胞反应中。
在这里，我们研究了 R-Ras 在过敏原诱导的免疫反应（2 型免疫反应）中在 Rras 缺陷（R-Ras KO）和野生型（WT）小鼠中的作用。
最初，我们发现 R-Ras KO 小鼠淋巴结（LNs）中的常规 DC 数量减少。这些细胞上的共刺激分子 CD80 和 CD86 的表达也减少了。然而，在免疫后，通过血清 IgE 水平测量，R-Ras WT 和 KO 之间的木瓜蛋白酶诱导的免疫反应没有差异。有趣的是，在免疫后，WT 和 R-Ras KO 动物之间的 DC 数量或共刺激分子表达没有差异。
尽管 R-Ras KO 小鼠中的常规 DC 数量减少，且 DC 上的 CD80 表达降低，但 R-Ras KO 小鼠能够引发类似于 WT 小鼠的木瓜蛋白酶诱导的 IgE 反应。据我们所知，这是第一个报道 R-Ras GTP 酶调节体内变应原反应差异的报告。
