Institute of Cardiovascular Research and Sports Medicine, Department of Molecular and Cellular Sports Medicine, German Sport University Cologne, Germany.
Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut, Karlsruhe, Germany.
Mol Nutr Food Res. 2017 Oct;61(10). doi: 10.1002/mnfr.201600843. Epub 2017 Jul 3.
This study was to investigate anabolic adaptation of skeletal muscle in response to an isoflavone (ISO) enriched diet, training and their combinations in ovariectomized (OVX) rats.
Female Wistar rats were sedentary, performed treadmill uphill running, received ISOs, or a combination of ISOs and running after ovariectomy. Body weight was increased by OVX. Both ISO and training treatment antagonized this increase. The weights of soleus and gastrocnemius muscles were increased only when training and ISOs were combined. In soleus muscle insulin-like growth factor (IGF)-1R, MyoD and Myogenin expressions were only up-regulated by training in Sham groups. However, a stimulation of IGF-1R and MyoD expression could be observed when ISOs and training were combined. In gastrocnemius muscle MyoD and Myogenin expressions were stimulated by either training or ISOs. Additive effects were detected when combining the two interventions.
Our results indicate that the combination of ISOs and exercise is more efficient in increasing relative skeletal muscle mass and the expression of molecular markers related to anabolic adaptation in the skeletal muscle of female rats.
本研究旨在探讨富含异黄酮（ISO）的饮食、运动及其联合应用对去卵巢大鼠骨骼肌合成代谢适应的影响。
雌性 Wistar 大鼠为安静组，进行跑步机爬坡运动，接受 ISO 或 ISO 和运动的联合处理，去卵巢后大鼠体重增加。ISO 和运动处理均拮抗了这种增加。只有在运动和 ISO 联合应用时，比目鱼肌和腓肠肌的重量才会增加。在 Sham 组中，只有运动能上调比目鱼肌中胰岛素样生长因子（IGF）-1R、MyoD 和 Myogenin 的表达。然而，当 ISO 和运动联合应用时，IGF-1R 和 MyoD 的表达可以被刺激。在腓肠肌中，运动或 ISO 都能刺激 MyoD 和 Myogenin 的表达。两种干预措施联合应用时，可检测到累加效应。
我们的结果表明，ISO 和运动的联合应用更有效地增加了雌性大鼠骨骼肌的相对质量和与合成代谢适应相关的分子标志物的表达。
