基于Taqman的实时荧光定量PCR检测方法的建立，用于快速、特异性检测新型鸭源鹅细小病毒。
Development of a taqman-based real-time PCR assay for the rapid and specific detection of novel duck- origin goose parvovirus.
作者信息
Wang Jianchang, Wang Jinfeng, Cui Yuan, Nan Huizhu, Yuan Wanzhe
机构信息
Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, Hebei 050051, China.
College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China.
出版信息
Mol Cell Probes. 2017 Aug;34:56-58. doi: 10.1016/j.mcp.2017.05.001. Epub 2017 May 9.
A real-time PCR assay was developed for specific detection of novel duck-origin goose parvovirus (N-GPV), the etiological agent of duck beak atrophy and dwarfism syndrome (BADS). The detection limit of the assay was 10 copies. The assay was useful in the prevention and control of BADS.
建立了一种实时荧光定量PCR检测方法，用于特异性检测新型鸭源鹅细小病毒（N-GPV），它是鸭喙萎缩和侏儒综合征（BADS）的病原体。该检测方法的检测限为10个拷贝。该方法对BADS的防控具有重要意义。
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