建立一种基于双重 SYBR Green I 的实时荧光定量 PCR 方法，用于快速区分鹅细小病毒和麝香鸭细小病毒。
Development of a duplex SYBR Green I-based quantitative real-time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses.
机构信息
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, 350003, China.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, 350013, China.
出版信息
Virol J. 2019 Jan 10;16(1):6. doi: 10.1186/s12985-018-1111-7.
BACKGROUND
Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.
RESULTS
A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.
CONCLUSION
This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.
背景
水禽细小病毒，包括鹅细小病毒（GPV）和麝香鸭细小病毒（MDPV），可导致鹅和鸭的严重疾病。开发针对这两种细小病毒的快速、准确诊断方法尤为重要。
结果
建立了一种基于双重 SYBR Green I 的实时荧光定量 PCR 检测方法，用于同时检测和区分 GPV 和 MDPV。当评估 GPV 或 MDPV 时，该方法分别产生具有特异性单峰（Tm = 87.3 ± 0.26°C 或 Tm = 85.4 ± 0.23°C）的熔解曲线。当在一个反应中同时评估两种细小病毒时，会产生具有特异性双峰的熔解曲线。
结论
该双重实时 RT-PCR 可用于快速鉴定现场病例和人工试验中的 GPV 和 MDPV，使其成为诊断、预防和控制水禽细小病毒感染的有力工具。
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