Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, 350003, China.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, 350013, China.
Virol J. 2019 Jan 10;16(1):6. doi: 10.1186/s12985-018-1111-7.
Waterfowl parvoviruses, including goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), can cause seriously diseases in geese and ducks. Developing a fast and precise diagnosis assay for these two parvoviruses is particularly important.
A duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and MDPV. The assay yielded melting curves with specific single peak (Tm = 87.3 ± 0.26 °C or Tm = 85.4 ± 0.23 °C) when GPV or MDPV was evaluated, respectively. When both parvoviruses were assessed in one reaction, melting curves with specific double peaks were yielded.
This duplex quantitative RT-PCR can be used to rapid identify of GPV and MDPV in field cases and artificial trials, which make it a powerful tool for diagnosing, preventing and controlling waterfowl parvovirus infections.
水禽细小病毒，包括鹅细小病毒（GPV）和麝香鸭细小病毒（MDPV），可导致鹅和鸭的严重疾病。开发针对这两种细小病毒的快速、准确诊断方法尤为重要。
建立了一种基于双重 SYBR Green I 的实时荧光定量 PCR 检测方法，用于同时检测和区分 GPV 和 MDPV。当评估 GPV 或 MDPV 时，该方法分别产生具有特异性单峰（Tm = 87.3 ± 0.26°C 或 Tm = 85.4 ± 0.23°C）的熔解曲线。当在一个反应中同时评估两种细小病毒时，会产生具有特异性双峰的熔解曲线。
该双重实时 RT-PCR 可用于快速鉴定现场病例和人工试验中的 GPV 和 MDPV，使其成为诊断、预防和控制水禽细小病毒感染的有力工具。
