Thanchomnang Tongjit, Intapan Pewpan M, Sanpool Oranuch, Rodpai Rutchanee, Tourtip Somjintana, Yahom Sujitra, Kullawat Jitsuda, Radomyos Prayong, Thammasiri Chalida, Maleewong Wanchai
Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.
Research and Diagnostic Center for Emerging Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Parasitol Res. 2017 Jul;116(7):1917-1923. doi: 10.1007/s00436-017-5469-z. Epub 2017 May 12.
The parasitic nematodes, Strongyloides stercoralis and Strongyloides fuelleborni, can infect humans and non-human primates. We amplified and sequenced a portion of the 18S ribosomal RNA gene (rRNA) and of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of Strongyloides from humans in the study area in Thailand, where people have frequent contact with long-tailed macaques. Fresh stool samples were obtained from 213 people and were examined using the agar plate culture method. The overall prevalence of Strongyloides infection was 8.92% (19/213). From a total of 19 worms (one per infected person), 18 adult males had 18S rRNA sequences identical with that of S. stercoralis and one adult female had a sequence almost identical with that of S. fuelleborni. A median-joining network of cox1 sequences revealed nine new haplotypes from S. stercoralis, and an overall haplotype diversity (Hd) of 0.9309. The single haplotype of S. fuelleborni was also new and contributed to an overall haplotype diversity for that species of 0.9842. This is the first molecular identification of S. stercoralis and S. fuelleborni in a human community having contact with long-tailed macaques in Thailand. It is also the first report of S. fuelleborni infecting a human in Thailand.
寄生线虫粪类圆线虫和弗氏类圆线虫可感染人类和非人类灵长类动物。我们对泰国研究区域内与长尾猕猴频繁接触的人群中类圆线虫的18S核糖体RNA基因（rRNA）和线粒体细胞色素c氧化酶亚基1（cox1）基因的部分片段进行了扩增和测序。从213人身上采集了新鲜粪便样本，并采用琼脂平板培养法进行检测。类圆线虫感染的总体患病率为8.92%（19/213）。在总共19条线虫（每个感染者1条）中，18条成年雄性线虫的18S rRNA序列与粪类圆线虫相同，1条成年雌性线虫的序列与弗氏类圆线虫几乎相同。cox1序列的中位数连接网络显示，粪类圆线虫有9个新单倍型，总体单倍型多样性（Hd）为0.9309。弗氏类圆线虫的单一单倍型也是新的，该物种的总体单倍型多样性为0.9842。这是泰国与长尾猕猴接触的人类群体中首次对粪类圆线虫和弗氏类圆线虫进行分子鉴定。这也是泰国首次报道弗氏类圆线虫感染人类。
