da Silva Marcos Vinícius, Marosti Aline Rosa, Mendes Cristina Eusébio, Palombit Kelly, Castelucci Patricia
Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Brazil; University Federal of Sergipe, Brazil.
Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Brazil.
Acta Histochem. 2017 Jun;119(5):481-494. doi: 10.1016/j.acthis.2017.05.001. Epub 2017 May 11.
The aim of this study was to evaluate the effect of ulcerative colitis on the submucosal neurons and glial cells of the submucosal ganglia of rats. 2,4,6-Trinitrobenzene sulfonic acid (TNBS; colitis group) was administered in the colon to induce ulcerative colitis, and distal colons were collected after 24h. The colitis rats were compared with those in the sham and control groups. Double labelling of the P2X7 receptor with calbindin (marker for intrinsic primary afferent neurons, IPANs, submucosal plexus), calretinin (marker for secretory and vasodilator neurons of the submucosal plexus), HuC/D and S100β was performed in the submucosal plexus. The density (neurons per area) of submucosal neurons positive for the P2X7 receptor, calbindin, calretinin and HuC/D decreased by 21%, 34%, 8.2% and 28%, respectively, in the treated group. In addition, the density of enteric glial cells in the submucosal plexus decreased by 33%. The profile areas of calbindin-immunoreactive neurons decreased by 25%. Histological analysis revealed increased lamina propria and decreased collagen in the colitis group. This study demonstrated that ulcerative colitis affected secretory and vasodilatory neurons, IPANs and enteric glia of the submucosal plexus expressing the P2X7 receptor.
本研究的目的是评估溃疡性结肠炎对大鼠黏膜下神经节的黏膜下神经元和神经胶质细胞的影响。将2,4,6-三硝基苯磺酸（TNBS；结肠炎组）注入结肠以诱导溃疡性结肠炎，24小时后收集结肠远端。将结肠炎大鼠与假手术组和对照组大鼠进行比较。在黏膜下神经丛中对P2X7受体与钙结合蛋白（内在初级传入神经元、IPANs、黏膜下丛的标志物）、钙视网膜蛋白（黏膜下丛的分泌和血管舒张神经元的标志物）、HuC/D和S100β进行双重标记。在治疗组中，P2X7受体、钙结合蛋白、钙视网膜蛋白和HuC/D阳性的黏膜下神经元密度（每单位面积的神经元数量）分别下降了21%、34%、8.2%和28%。此外，黏膜下神经丛中肠神经胶质细胞的密度下降了33%。钙结合蛋白免疫反应性神经元的轮廓面积减少了25%。组织学分析显示，结肠炎组的固有层增加，胶原蛋白减少。本研究表明，溃疡性结肠炎影响了表达P2X7受体的黏膜下神经丛的分泌和血管舒张神经元、IPANs和肠神经胶质细胞。
