Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People's Republic of China.
Arch Microbiol. 2013 Dec;195(12):815-22. doi: 10.1007/s00203-013-0930-6. Epub 2013 Oct 20.
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3(T), was isolated from a rice paddy soil in South China. Growth occurred with 0-3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5-9.0 (optimum pH 7.0) and at 25-42 °C (optimum 30-37 °C) in liquid Reasoner's 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3(T) was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701(T) and P. sagittaria JCM 18195(T) were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA-DNA hybridization between strain WM-3(T) and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C16:0 (30.0 %), C16:1 ω6c and/or C16:1 ω7c (19.3 %) and C18:1 ω6c and/or C18:1 ω7c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3(T) belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3(T) (=KCTC 32247(T) =CGMCC 1.12417(T)).
一株革兰氏阴性、杆状、运动型、具多根极生鞭毛的细菌，被命名为 WM-3(T)，从中国南方稻田土壤中分离得到。在液体 Reasoner 2A 培养基中，该菌在 0-3.0%（w/v）NaCl（最佳 2.0%）、pH5.5-9.0（最佳 pH7.0）和 25-42°C（最佳 30-37°C）的条件下生长。16S rRNA 基因和 gyrB 基因序列分析表明，WM-3(T) 菌株与种 Pseudomonas linyingensis 和 Pseudomonas sagittaria 的模式菌株最为密切相关。其与 P. linyingensis CGMCC 1.10701(T) 和 P. sagittaria JCM 18195(T) 的 16S rRNA 基因序列相似性分别为 97.4%和 97.3%，gyrB 基因序列相似性分别为 94.1%和 94.2%。WM-3(T) 菌株与这两个模式菌株之间的 DNA-DNA 杂交显示出 35.6%和 30.9%的相关性。基因组 DNA 的 G+C 含量为 69.4 mol%。全细胞脂肪酸主要由 C16:0（30.0%）、C16:1 ω6c 和/或 C16:1 ω7c（19.3%）和 C18:1 ω6c 和/或 C18:1 ω7c（16.3%）组成。表型、化学分类和基因型分析的结果清楚地表明，WM-3(T) 菌株属于假单胞菌属，但代表了一个新的种，建议将其命名为 Pseudomonas oryzae sp. nov. 。模式菌株为 WM-3(T)=KCTC 32247(T)=CGMCC 1.12417(T)。
