Eom Min Rye, Weon Jin Bae, Jung Youn Sik, Ryu Ga Hee, Yang Woo Seung, Ma Choong Je
Department of Medical Biomaterials Engineering, College of Biomedical science, Kangwon National University, Hyoja-2 Dong, Chuncheon, 200-701, Republic of Korea.
Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Republic of Korea.
Arch Pharm Res. 2017 Jun;40(6):704-712. doi: 10.1007/s12272-017-0918-x. Epub 2017 May 13.
Glutamate is a neurotransmitter in central nervous system. Overexpression of glutamate leads to oxidative stress, resulting in several neurodegenerative disorders that include Alzheimer's disease. The n-hexane fraction of stems and ethyl acetate (EtOAc) fraction of flowers of Reynoutria sachalinensis provide neuroprotection against glutamate-induced oxidative toxicity in HT22 cells. In this study, 1-decanol (1), β-amyrin (2), dammaran-3β-ol (3), campesterol (4), daucosterol (5), ergosterol peroxide (6), emodin 8-O-β-D-glucopyranoside (7), quercetin (8) and isoquercitrin (9) were isolated from n-hexane fractions of stems and EtOAc fractions of flowers of R. sachalinensis. Their neuroprotective activity was evaluated by MTT assay. 1-Decanol, campesterol, ergosterol peroxide, quercetin and isoquercitrin exhibited neuroprotective activity. These compounds decreased reactive oxygen species level, showed anti-oxidant activity with DPPH radical and in a HO scavenging assay. Therefore, the neuroprotective activity of 1-decanol, campesterol, ergosterol peroxide, quercetin and isoquercitrin are associated with antioxidant activity.
谷氨酸是中枢神经系统中的一种神经递质。谷氨酸的过度表达会导致氧化应激，引发包括阿尔茨海默病在内的多种神经退行性疾病。虎杖茎的正己烷馏分和花的乙酸乙酯（EtOAc）馏分对HT22细胞中谷氨酸诱导的氧化毒性具有神经保护作用。在本研究中，从虎杖茎的正己烷馏分和花的乙酸乙酯馏分中分离出了1-癸醇（1）、β-香树脂醇（2）、达玛烷-3β-醇（3）、菜油甾醇（4）、胡萝卜苷（5）、过氧化麦角甾醇（6）、大黄素8-O-β-D-葡萄糖苷（7）、槲皮素（8）和异槲皮苷（9）。通过MTT法评估了它们的神经保护活性。1-癸醇、菜油甾醇、过氧化麦角甾醇、槲皮素和异槲皮苷表现出神经保护活性。这些化合物降低了活性氧水平，在DPPH自由基清除试验和HO清除试验中显示出抗氧化活性。因此，1-癸醇、菜油甾醇、过氧化麦角甾醇、槲皮素和异槲皮苷的神经保护活性与抗氧化活性相关。
