Kim Brianna
Ceres Nanosciences, Inc., 9385 Discovery Blvd, Manassas, VA, 20110, USA.
Methods Mol Biol. 2017;1606:133-139. doi: 10.1007/978-1-4939-6990-6_9.
The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA), a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.
蛋白质印迹法是一项重要的实验室技术，可用于蛋白质的特异性鉴定和特性分析。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离的蛋白质通过电泳转移至聚偏二氟乙烯（PVDF）膜上，然后与特异性抗体一起孵育，随后显影以显示目标蛋白质。在此，我们描述了外表面蛋白A（OspA）的转移和检测，OspA是一种仅在伯氏疏螺旋体表面发现的蛋白质，伯氏疏螺旋体是引发莱姆病的细菌。
