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基质固相分散作为植物化学和生物活性表征工具:山楂的案例研究。

Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha L._A case study.

作者信息

Benabderrahmane Wassila, Lores Marta, Lamas Juan Pablo, Benayache Samir

机构信息

a Unit of Valorization of Natural Resources, bioactive molecules and physicochemical and biological analysis (VARENBIOMO), Faculty of Science , University of Constantine 1 , Constantine , Algeria.

b Laboratory of Research and Development of Analytical Solutions (LIDSA), Faculty of Chemistry, Analytical Chemistry Department , Campus VIDA-USC , Santiago de Compostela , Spain.

出版信息

Nat Prod Res. 2018 May;32(10):1220-1223. doi: 10.1080/14786419.2017.1326040. Epub 2017 May 15.

DOI:10.1080/14786419.2017.1326040
PMID:28503940
Abstract

The use of a matrix solid-phase dispersion (MSPD) process to extract polyphenols from hawthorn (Crataegus oxyacantha L.) a deciduous shrub with an expected rich phytochemical profile, has been evaluated. MSPD extracts of fruits and leaves have an outstanding content of polyphenols, although the particular phenolic profile is solvent dependent. The extracts were analysed by HPLC-DAD for the accurate identification of the major bioactive polyphenols, some of which have never been described for this species. MSPD has proven to be a good alternative to the classic methods of obtaining natural extracts, fast and with low consumption of organic solvents, therefore, environmentally friendly. The bioactivities can be considered also very remarkable, revealing extracts with high levels of antioxidant activity.

摘要

已对采用基质固相分散(MSPD)工艺从山楂(Crataegus oxyacantha L.)中提取多酚进行了评估,山楂是一种落叶灌木,预计含有丰富的植物化学物质。果实和叶片的MSPD提取物中多酚含量很高,不过特定的酚类成分取决于溶剂。通过高效液相色谱-二极管阵列检测法(HPLC-DAD)对提取物进行分析,以准确鉴定主要的生物活性多酚,其中一些多酚从未在该物种中被描述过。事实证明,MSPD是获取天然提取物的经典方法的良好替代方法,速度快且有机溶剂消耗低,因此对环境友好。提取物的生物活性也非常显著,显示出具有高水平的抗氧化活性。

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