Benabderrahmane Wassila, Lores Marta, Benaissa Ouahiba, Lamas Juan Pablo, de Miguel Trinidad, Amrani Amel, Benayache Fadila, Benayache Samir
Unité de recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Département de Chimie, Faculté des Sciences Exactes, Université Constantine 1, Constantine, Algérie.
Laboratory of Research and Development of Analytical Solutions (LIDSA). Analytical Chemistry Department. Faculty of Chemistry, Campus VIDA-USC, Santiago de composela, Spain.
Nat Prod Res. 2021 Feb;35(4):627-632. doi: 10.1080/14786419.2019.1582044. Epub 2019 Mar 5.
Solid-Liquid Extraction (SLE) using solvent of different polarities (CHCl, EtOAc, and -BuOH) has been applied to leaves and fruits from ( L.), a deciduous shrub with an expected rich phytochemical profile. The total polyphenols content and the radical scavenging activity of each extract were evaluated. These extracts were analyzed by HPLC-DAD and rutin, quercetin-3-glucoside, caftaric and caffeic acid had been positively identified. The phytochemical study of the ethyl acetate extract of led to the isolation and structural elucidation of quercetin (1); quercetin-3--β-glucoside (2); epicatechin (3); naringenin (4), reported for the first time from this species except caffeic acid and epicatechin. These compounds were identified by 1D and 2D NMR combined analysis as well as by MS and UV.The antimicrobial activity of these extracts has also been tested, showing strong antibacterial activity-solvent dependent-against Gram positive bacteria. Additionally, bactericidal power was demonstrated in fruit extracts.
使用不同极性的溶剂（氯仿、乙酸乙酯和正丁醇）进行固液萃取（SLE），已应用于落叶灌木（此处拉丁名缺失）的叶子和果实，该灌木预计具有丰富的植物化学特征。对每种提取物的总多酚含量和自由基清除活性进行了评估。通过HPLC-DAD对这些提取物进行分析，已明确鉴定出芦丁、槲皮素-3-葡萄糖苷、咖啡酰酒石酸和咖啡酸。对该植物乙酸乙酯提取物的植物化学研究导致了槲皮素（1）、槲皮素-3-β-葡萄糖苷（2）、表儿茶素（3）、柚皮素（4）的分离和结构解析，除咖啡酸和表儿茶素外，这些化合物首次从该物种中报道。这些化合物通过一维和二维核磁共振联合分析以及质谱和紫外光谱进行鉴定。还测试了这些提取物的抗菌活性，结果表明其对革兰氏阳性菌具有强烈的溶剂依赖性抗菌活性。此外，果实提取物表现出杀菌能力。
