Kirakosyan Ara, Seymour Elisabeth, Kaufman Peter B, Warber Sara, Bolling Steven, Chang Soo Chul
Department of Molecular Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA.
J Agric Food Chem. 2003 Jul 2;51(14):3973-6. doi: 10.1021/jf030096r.
Crataegus laevigata and Crataegus monogyna (hawthorn) were subjected to drought and cold stress treatments, and polyphenolic extracts from control and stress-treated plants were assayed for antioxidant capacities using a modified version of the Total Antioxidant Status Assay (Randox, San Francisco, CA). In addition, these plants were analyzed for levels of flavanol-type substance [(-)-epicatechin] and flavonoid (vitexin 2' '-O-rhamnoside, acetylvitexin 2' '-O-rhamnoside, and hyperoside) constituents that are important metabolites in hawthorn herbal preparations used to treat patients with heart disease. Drought and cold stress treatments caused increases in levels of (-)-epicatechin and hyperoside in both Crataegus species. Such treatments also enhanced the antioxidant capacity of the extracts. The results from this study thus indicate that these kinds of stress treatments can enhance the levels of important secondary metabolites and their total antioxidant capacities in leaves of Crataegus.
对光叶山楂和山楂（山楂属）进行干旱和低温胁迫处理，使用改良版总抗氧化状态测定法（兰多克斯公司，加利福尼亚州旧金山）对对照植株和胁迫处理植株的多酚提取物进行抗氧化能力测定。此外，分析这些植株中黄烷醇类物质[（-）-表儿茶素]和黄酮类化合物（牡荆素2''-O-鼠李糖苷、乙酰基牡荆素2''-O-鼠李糖苷和金丝桃苷）成分的含量，这些成分是用于治疗心脏病患者的山楂草药制剂中的重要代谢产物。干旱和低温胁迫处理导致两种山楂属植物中（-）-表儿茶素和金丝桃苷的含量增加。此类处理还增强了提取物的抗氧化能力。因此，本研究结果表明，这些胁迫处理可提高山楂叶片中重要次生代谢产物的含量及其总抗氧化能力。
