Bang Sunghee, Li Wei, Ha Thi Kim Quy, Lee Changyeol, Oh Won Keun, Shim Sang Hee
a College of Pharmacy , Duksung Women's University , Seoul , Republic of Korea.
b Korean Medicine (KM) Application Center , Korea Institute of Oriental Medicine , Daegu , Republic of Korea.
Nat Prod Res. 2018 May;32(10):1224-1228. doi: 10.1080/14786419.2017.1326042. Epub 2017 May 15.
To determine the compounds responsible for its anti-influenza activities, we isolated the three flavonoids, 6-hydroxyluteolin 7-O-β-d-glucoside (1), nepitrin (2), homoplantaginin (3) from the MeOH extract of Salvia plebeia R.Br. and identified them by comparing the spectroscopic data with that reported in the literature. The contents of the three flavonoids in the whole extract were 108.74 ± 0.95, 46.26 ± 2.19, and 69.35 ± 1.22 mg/g for 6-hydroxyluteolin 7-O-β-d-glucoside, nepitrin, and homoplantaginin, respectively, which demonstrates that they are the major constituents of this plant. The three flavonoids were evaluated for their inhibitory activities against influenza virus H1N1 A/PR/9/34 neuraminidase and H1N1-induced cytopathic effect (CPE) on Madin-Darby canine kidney (MDCK) cells. Our results demonstrated the following arrangement for their anti-influenza activities: nepitrin (2) > 6-hydroxyluteolin 7-O-β-d-glucoside (1) > homoplantaginin (3). The potent inhibitory activities of these flavonoids against influenza suggested their potential to be developed as novel anti-influenza drugs in the future.
为确定具有抗流感活性的化合物，我们从荔枝草的甲醇提取物中分离出三种黄酮类化合物，即6-羟基木犀草素7-O-β-D-葡萄糖苷（1）、尼泊尔黄酮苷（2）、高车前苷（3），并通过将光谱数据与文献报道的数据进行比较来鉴定它们。在整个提取物中，这三种黄酮类化合物的含量分别为：6-羟基木犀草素7-O-β-D-葡萄糖苷108.74±0.95mg/g、尼泊尔黄酮苷46.26±2.19mg/g、高车前苷69.35±1.22mg/g，这表明它们是该植物的主要成分。对这三种黄酮类化合物针对甲型流感病毒H1N1 A/PR/9/34神经氨酸酶以及对Madin-Darby犬肾（MDCK）细胞的H1N1诱导的细胞病变效应（CPE）的抑制活性进行了评估。我们的结果表明它们的抗流感活性顺序如下：尼泊尔黄酮苷（2）>6-羟基木犀草素7-O-β-D-葡萄糖苷（1）>高车前苷（3）。这些黄酮类化合物对流感的强效抑制活性表明它们未来有开发成为新型抗流感药物的潜力。
