Li Xianping, Wang Mingzhe, Liu Chang, Xiao Yuchun, Li Mengde, Ban Chengjun, Huang Yuanming, Cheng Miao, Song Liqiong, Liu Guoxing, Lu Shan, Wang Chengxiang, Ren Zhihong
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018RU010), Chinese Academy of Medical Sciences, Beijing, China.
Respiratory Department, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine (BUCM), Beijing, China.
Front Pharmacol. 2022 May 23;13:874068. doi: 10.3389/fphar.2022.874068. eCollection 2022.
Influenza virus-caused lung infection and its pandemic outbreaks are a persistent public health challenge. The H1N1 subtype is the most common type of influenza infection observed in humans. Maxingshigantang decoction, a classic formula of Chinese herbal medicine, has been used for the prevention and treatment of respiratory infection for many centuries. Qingfeiyin decoction, based on Maxingshigantang, has been used in the clinic for decades. To explore the underlying mechanisms, according to the traditional Chinese medicine theory "the lung and the large intestine are interior-exterior," which can be translated to the "gut-lung axis" in a contemporary term, the composition of gut microbiota was determined using 16S rRNA and the transcriptome of the colon was determined by RNA sequencing. The results showed that Qingfeiyin decoction decreased the viral load, alleviated the lung injury, increased the survival rate, partly restored the shortening of the colon caused by the H1N1 virus, and downregulated inflammatory pathways including MAPK, TNFα, and JAK-STAT signaling pathways. Qingfeiyin decoction increased the relative abundance of the genera of , and and prevented the H1N1 virus-induced decrease in the abundance of the genera of , , and These results will help better understand the mechanisms for Qingfeiyin decoction's protective effect against influenza virus infection.
流感病毒引起的肺部感染及其大流行爆发是一个持续存在的公共卫生挑战。H1N1亚型是人类中最常见的流感感染类型。麻杏石甘汤是一种经典的中药方剂，数百年来一直用于预防和治疗呼吸道感染。基于麻杏石甘汤的清肺饮已在临床上使用了数十年。为了探究其潜在机制，根据中医理论“肺与大肠相表里”，在当代可将其理解为“肠-肺轴”，采用16S rRNA测定肠道微生物群的组成，并通过RNA测序测定结肠的转录组。结果表明，清肺饮降低了病毒载量，减轻了肺损伤，提高了存活率，部分恢复了由H1N1病毒引起的结肠缩短，并下调了包括MAPK、TNFα和JAK-STAT信号通路在内的炎症途径。清肺饮增加了[具体菌属名称1]、[具体菌属名称2]和[具体菌属名称3]菌属的相对丰度，并防止了H1N1病毒诱导的[具体菌属名称4]、[具体菌属名称5]、[具体菌属名称6]和[具体菌属名称7]菌属丰度的降低。这些结果将有助于更好地理解清肺饮对流感病毒感染的保护作用机制。 （注：原文中部分菌属名称缺失，翻译时用[具体菌属名称]表示）
