Ong Oselyne T W, Green-Barber Jai M, Kanuri Anusha, Young Lauren J, Old Julie M
School of Science and Health, Hawkesbury, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
School of Science and Health, Hawkesbury, University of Western Sydney, Locked Bag 1797, Penrith, NSW 2751, Australia.
Comp Immunol Microbiol Infect Dis. 2017 Apr;51:41-48. doi: 10.1016/j.cimid.2017.03.001. Epub 2017 Mar 29.
Antimicrobial substances in serum include circulating complement proteins and acute phase proteins (APPs). We identified gene sequences for APPs, haptoglobin (Hp), C-reactive protein (CRP) and serum amyloid A (SAA) in marsupial genomes. Hp and SAA levels were measured in red-tailed phascogale (Phascogale calura) sera using commercially available assays. Hp levels were higher in males than females, while SAA levels suggest the phascogales used in this study were healthy. Serum was co-cultured with four bacterial species. Bacterial growth was inhibited after incubation at 37°C, however effectiveness differed with bacteria and incubation time. The least amount of bacterial growth was noticed after introduction to K. pneumoniae, and most when introduced to P. aeruginosa. Despite marsupials not having mature immune tissues at birth, and unable to mount specific immune responses, this study suggests other immune strategies, such as APPs in serum likely aid marsupials in their defence against pathogens.
血清中的抗菌物质包括循环补体蛋白和急性期蛋白（APPs）。我们在有袋类动物基因组中鉴定出了APPs、触珠蛋白（Hp）、C反应蛋白（CRP）和血清淀粉样蛋白A（SAA）的基因序列。使用市售检测方法测定了红尾袋鼬（Phascogale calura）血清中的Hp和SAA水平。雄性的Hp水平高于雌性，而SAA水平表明本研究中使用的袋鼬健康。将血清与四种细菌共同培养。在37°C孵育后细菌生长受到抑制，然而其有效性因细菌和孵育时间而异。引入肺炎克雷伯菌后观察到的细菌生长量最少，引入铜绿假单胞菌时生长量最多。尽管有袋类动物出生时没有成熟的免疫组织，也无法产生特异性免疫反应，但本研究表明其他免疫策略，如血清中的APPs可能有助于有袋类动物抵御病原体。
