School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
Carbohydr Polym. 2017 Aug 1;169:157-166. doi: 10.1016/j.carbpol.2017.03.082. Epub 2017 Mar 29.
A novel core-shell structured nanofilm for the delivery of protein to the colon was developed by coaxial electrospinning using bovine serum albumin (BSA) as protein model. Firstly, the BSA-loaded chitosan nanoparticle was prepared by ionic gelation, and then the coaxial nanofilm was fabricated using alginate as shell layer and the BSA-loaded chitosan nanoparticle as core layer. Scanning electron microscopy analysis showed that the obtained nanofilm exhibited a smooth surface, and the core-shell structure was evidenced by the aid of transmission electron microscopy. There was little change in the secondary structure of encapsulated BSA and around 75% of BSA was released in the simulated colonic fluid. The corresponding kinetics models of BSA release in different simulated digestive fluids were built and the results revealed that the release of BSA in colon followed a complex mechanism. This study shows that electrospun nanofilm is a promising colon-specific delivery system for bioactive protein.
采用同轴静电纺丝技术，以牛血清白蛋白（BSA）为蛋白模型，制备了一种新型的用于结肠递药的核壳结构纳米纤维。首先，通过离子凝胶化制备载 BSA 的壳聚糖纳米颗粒，然后以海藻酸钠为壳层，载 BSA 的壳聚糖纳米颗粒为核层，制备同轴纳米纤维。扫描电子显微镜分析表明，所得到的纳米纤维具有光滑的表面，并且通过透射电子显微镜证实了核壳结构的存在。包封的 BSA 的二级结构几乎没有变化，并且约有 75%的 BSA 在模拟结肠液中释放。在不同模拟消化液中构建了 BSA 释放的相应动力学模型，结果表明 BSA 在结肠中的释放遵循复杂的机制。本研究表明，静电纺纳米纤维是一种有前途的用于生物活性蛋白的结肠特异性递药系统。
