Wen Peng, Wen Yan, Huang Xiao, Zong Min-Hua, Wu Hong
School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
J Agric Food Chem. 2017 Jun 14;65(23):4786-4796. doi: 10.1021/acs.jafc.7b01830. Epub 2017 Jun 2.
For the enhancement of protein's bioavailability, a specific delivery system was developed by coaxial electrospinning. Bovine serum albumin (BSA) was used as protein model, and the core-sheath fiber mat was fabricated using sodium alginate as shell layer and the BSA-loaded chitosan nanoparticle that was prepared previously as core layer. By optimizing electrospinning parameters, uniform fibers with diameters ranging from 200-600 nm were obtained, and transmission electron microscopy and confocal laser scanning microscopy revealed their core-sheath structures. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated that there existed molecular interaction between components, which enhanced the mat's thermal stability and mechanic property. It was found that the predominant release mechanism of BSA from fiber mat was erosion, and little change occurred in the secondary structure of encapsulated BSA indicated by FTIR and circular dichroism analysis. The study shows that the obtained fiber mat is a potential delivery system for protein.
为提高蛋白质的生物利用度，通过同轴静电纺丝开发了一种特定的递送系统。以牛血清白蛋白（BSA）作为蛋白质模型，以海藻酸钠为壳层、先前制备的负载BSA的壳聚糖纳米颗粒为芯层制备了核壳纤维毡。通过优化静电纺丝参数，获得了直径范围为200-600nm的均匀纤维，透射电子显微镜和共聚焦激光扫描显微镜揭示了它们的核壳结构。傅里叶变换红外光谱（FTIR）分析表明各组分之间存在分子相互作用，这提高了纤维毡的热稳定性和机械性能。发现BSA从纤维毡中的主要释放机制是侵蚀，FTIR和圆二色性分析表明包封的BSA二级结构几乎没有变化。该研究表明，所获得的纤维毡是一种潜在的蛋白质递送系统。
