Vakilian Saeid, Mashayekhan Shohreh, Shabani Iman, Khorashadizadeh Mohsen, Fallah Ali, Soleimani Masoud
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran 11365-8639, Iran; Department of Nanotechnology and Tissue Engineering, Stem Cell Technology Research Center, Tehran 1997775555, Iran.
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran 11365-8639, Iran.
Int J Biol Macromol. 2015 Apr;75:248-57. doi: 10.1016/j.ijbiomac.2015.01.051. Epub 2015 Feb 4.
The cellular microenvironment can be engineered through the utilization of various nano-patterns and matrix-loaded bioactive molecules. In this study, a multilayer system of electrospun scaffold containing chitosan nanoparticles was introduced to overcome the common problems of instability and burst release of proteins from nanofibrous scaffolds. Bovine serum albumin (BSA)-loaded chitosan nanoparticles was fabricated based on ionic gelation interaction between chitosan and sodium tripolyphosphate. Suspension electrospinning was employed to fabricate poly-ɛ-caprolacton (PCL) containing protein-loaded chitosan nanoparticles with a core-shell structure. To obtain the desired scaffold mechanical properties with enough elasticity for expansion and contraction, a hybrid mono and multilayer electrospun scaffold was fabricated using PCL containing protein-loaded chitosan nanoparticles and poly-L-lactic acid (PLLA). According to the BSA release profile, the multi-layered structure of nanofibers with two barrier layers provided a programmable release pattern of the loaded protein. Moreover, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism spectra results showed that the electrospinning process had no significant effect on the primary and secondary structure of the protein. The results indicated a desirable biocompatibility and mechanical cues of the multilayer nanofibrous scaffolds supporting structural stability and controlled release of the protein, which can offer diverse applications in hollow organ tissue engineering.
细胞微环境可以通过利用各种纳米图案和负载基质的生物活性分子来构建。在本研究中，引入了一种含有壳聚糖纳米颗粒的电纺支架多层系统，以克服纳米纤维支架中蛋白质不稳定和突发释放的常见问题。基于壳聚糖与三聚磷酸钠之间的离子凝胶化相互作用，制备了负载牛血清白蛋白（BSA）的壳聚糖纳米颗粒。采用悬浮电纺技术制备了具有核壳结构、含有负载蛋白质的壳聚糖纳米颗粒的聚ε-己内酯（PCL）。为了获得具有足够弹性以进行扩张和收缩的所需支架机械性能，使用含有负载蛋白质的壳聚糖纳米颗粒的PCL和聚-L-乳酸（PLLA）制备了混合单层和多层电纺支架。根据BSA释放曲线，具有两个阻挡层的纳米纤维多层结构提供了负载蛋白质的可编程释放模式。此外，十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和圆二色光谱结果表明，电纺过程对蛋白质的一级和二级结构没有显著影响。结果表明，多层纳米纤维支架具有理想的生物相容性和机械线索，可支持蛋白质的结构稳定性和控释，这在中空器官组织工程中具有多种应用。
