Max von Pettenkofer-Institut Munich, Germany.
Bruker Daltonik GmbH Bremen, Germany.
Curr Issues Mol Biol. 2017;23:17-20. doi: 10.21775/cimb.023.017. Epub 2017 May 15.
Within less than a decade matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become a gold standard for microbial identification in clinical microbiology laboratories. Besides identification of microorganisms the typing of single strains as well as the antibiotic and antimycotic resistance testing has come into focus in order to speed up the microbiological diagnostic. However, the full potential of MALDI-TOF MS has not been tapped yet and future technological advancements will certainly expedite this method towards novel applications and enhancement of current practice. So, the following chapter shall be rather a brainstorming and forecast of how MALDI-TOF MS will develop to influence clinical diagnostics and microbial research in the future. It shall open up the stage for further discussions and does not claim for overall validity.
在不到十年的时间里，基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）已成为临床微生物学实验室中微生物鉴定的金标准。除了鉴定微生物外，单一菌株的分型以及抗生素和抗真菌药物耐药性检测也成为关注焦点，以加快微生物诊断。然而，MALDI-TOF MS 的全部潜力尚未被挖掘，未来的技术进步肯定会将这种方法应用于新的领域，并提高当前的实践水平。因此，以下章节更像是对 MALDI-TOF MS 将如何发展以影响未来临床诊断和微生物研究的头脑风暴和预测。它将为进一步的讨论开辟舞台，并不声称具有全面的有效性。
