Yue Xiaofeng, Que Yawei, Deng Shuzhen, Xu Lin, Oses-Ruiz Miriam, Talbot Nicholas J, Peng Youliang, Wang Zhengyi
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.
School of Biosciences, University of Exeter, Geoffrey Pope Building, Exeter, EX4 4QD, UK.
Environ Microbiol. 2017 Oct;19(10):3959-3981. doi: 10.1111/1462-2920.13796. Epub 2017 Jun 22.
Cell cycle regulation is pivotal for proper cell division and cellular differentiation in eukaryotic cells. The central regulators that govern eukaryotic cell cycle progression are cyclin-dependent kinases (CDKs) and their partners. Here, we report that Magnaporthe oryzae CKS1 encodes a cyclin-dependent kinase subunit, which plays a significant role in regulation of plant infection. We demonstrate that CKS1 is a functional homolog of CKS1/SUC1 and can physically interact with the CDK protein Cdc28, and Som1, a downstream regulator of the cyclic AMP-dependent Protein Kinase A pathway. CKS1 deletion mutants are severely impaired in hyphal growth, sexual reproduction, melanin pigmentation and conidiogenesis. Cks1 mutants are able to form appressoria from hyphal tips, but these are unable to re-polarize, and rice infection is impaired. CKS1 also affects chitin and glucan synthase activity during cell wall differentiation and fungal hydrophobin function. CKS1, therefore, encodes a conserved CDK-binding partner, essential for appressorium-mediated plant infection by the rice blast fungus.
细胞周期调控对于真核细胞的正常细胞分裂和细胞分化至关重要。控制真核细胞周期进程的核心调节因子是细胞周期蛋白依赖性激酶（CDK）及其伴侣。在此，我们报道稻瘟病菌CKS1编码一种细胞周期蛋白依赖性激酶亚基，其在植物感染调控中发挥重要作用。我们证明CKS1是CKS1/SUC1的功能同源物，并且可以与CDK蛋白Cdc28以及环磷酸腺苷依赖性蛋白激酶A途径的下游调节因子Som1发生物理相互作用。CKS1缺失突变体在菌丝生长、有性生殖、黑色素沉着和分生孢子形成方面严重受损。Cks1突变体能够从菌丝尖端形成附着胞，但这些附着胞无法重新极化，并且水稻感染受到损害。CKS1在细胞壁分化和真菌疏水蛋白功能过程中也影响几丁质和葡聚糖合酶活性。因此，CKS1编码一种保守的CDK结合伴侣，对于稻瘟病菌通过附着胞介导的植物感染至关重要。
