Nm-seq技术能够以碱基精度定位人类mRNA中的2'-O-甲基化位点。
Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.
作者信息
Dai Qing, Moshitch-Moshkovitz Sharon, Han Dali, Kol Nitzan, Amariglio Ninette, Rechavi Gideon, Dominissini Dan, He Chuan
机构信息
Department of Chemistry, The University of Chicago, Chicago, Illinois, USA.
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA.
出版信息
Nat Methods. 2017 Jul;14(7):695-698. doi: 10.1038/nmeth.4294. Epub 2017 May 15.
Abstract
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.
摘要
核糖核苷酸的核糖可被2'-O-甲基化（Nm）。尽管高通量检测取得了进展，但Nm的惰性化学性质仍然限制了灵敏度，并排除了在mRNA中的定位。我们利用2'-O-甲基化核苷和2'-羟基化核苷对高碘酸盐氧化的不同反应性，开发了Nm-seq，这是一种用于全转录组范围内以碱基精度定位Nm的灵敏方法。Nm-seq在人类mRNA中发现了数千个具有功能作用特征的Nm位点。
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