Assessment of Antimicrobial and Antioxidant Activities of Nepeta trachonitica: Analysis of Its Phenolic Compounds Using HPLC-MS/MS.

Continuing our work on the sources of natural bioactive compounds, we evaluated the antimicrobial and antioxidant activities of as well as its major phenolic content using the high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) technique. For antioxidant activity, ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) methods were performed to measure the reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed to evaluate the radical scavenging activity of the sample. For antimicrobial activity, three Gram-positive and four Gram-negative microbial species as well as three fungi species were tested. appeared to have reasonable antioxidant activity and decent antimicrobial activity as indicated by the inhibition of the organisms' growth. The most susceptible species were ATCC 6633 and ATCC 11229 among the organisms tested. Ethanol extract of the plant has the highest effect on but no effect on The HPLC-MS/MS analysis showed that at least 11 major phenolic compounds of exist, the major ones being rosmarinic acid, chlorogenic acid and quinic acid. The obtained results suggest that could be a promising source for food and nutraceutical industries because of its antimicrobial and antioxidant properties and phenolic compounds.