Briheim G, Stendahl O, Dahlgren C
Infect Immun. 1984 Jul;45(1):1-5. doi: 10.1128/iai.45.1.1-5.1984.
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. Luminol-dependent light emission from PMNL is linked to the myeloperoxidase (MPO)-H2O2 system. Light emission from a cell-free MPO-H2O2 system was found to be totally inhibited by human serum albumin (HSA), and since HSA is a large molecular protein that does not readily gain access to intracellular sites of PMNL, it could be used to determine the importance of extra- and intracellular events in PMNL chemiluminescence. In studies with cells from an MPO-deficient patient, we found that HSA inhibited more than 90% of extracellularly produced chemiluminescence. The chemotactic peptide formylmethionyl-leucyl-phenylalanine induced a two-peak chemiluminescence response in normal PMNL, and addition of HSA reduced the first peak, whereas the second peak was unaffected. This result indicated that the first peak was a result of extracellular reactions and the second peak was a result of intracellular reactions of the MPO-H2O2 system. Most of the phorbol myristate acetate-induced response in normal PMNL was due to intracellular events. Furthermore, chemiluminescence of intracellular origin seems to be limited not by generation of oxidative metabolites but by diffusion of luminol into the cells.
当多形核白细胞（PMNL）与可溶性或颗粒物质相互作用时，细胞会产生化学发光。PMNL中依赖鲁米诺的发光与髓过氧化物酶（MPO）-H2O2系统有关。发现无细胞MPO-H2O2系统的发光完全被人血清白蛋白（HSA）抑制，并且由于HSA是一种大分子蛋白质，不易进入PMNL的细胞内位点，因此它可用于确定细胞外和细胞内事件在PMNL化学发光中的重要性。在对一名MPO缺陷患者的细胞进行的研究中，我们发现HSA抑制了超过90%的细胞外产生的化学发光。趋化肽甲酰甲硫氨酰-亮氨酰-苯丙氨酸在正常PMNL中诱导了双峰化学发光反应，添加HSA降低了第一个峰，而第二个峰未受影响。这一结果表明，第一个峰是细胞外反应的结果，第二个峰是MPO-H2O2系统细胞内反应的结果。正常PMNL中大多数佛波酯诱导的反应是由于细胞内事件。此外，细胞内产生的化学发光似乎不是受氧化代谢产物生成的限制，而是受鲁米诺扩散到细胞内的限制。
