Armand Belal, Solhjoo Kavous, Kordshooli Manoochehr Shabani, Davami Mohammad Hasan, Pourahmad Morteza, Orfaee Vahideh
Department of Parasitology and Mycology, Jahrom University of Medical Sciences, Jahrom, Iran.
Department of Parasitic Disease, Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Vet World. 2017 Apr;10(4):386-392. doi: 10.14202/vetworld.2017.386-392. Epub 2017 Apr 7.
This study was performed to determine the genetic diversity of in sheep using nested-polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in Southern Iran.
The tissue samples of diaphragm and heart from 125 sheep were collected from the main slaughterhouses of Jahrom district in South of Fars province, Iran, between Aprils and June 2013. The DNA were extracted and analyzed by nested-PCR using specific primers for SAG2 and GRA6 loci. RFLP was used to classify strains into one of the three major lineages of .
Type I was predominant in this area. The data obtained from both loci demonstrated that the frequency of each genotype was 72% Type I, 2.4% Type III, 7.2% mixed Type I and II, 16.8% mixed Type I and III, 0.8% mixed Type II and III, and 0.8% mixed Type I, II and III.
Although the previously published data indicated that Type II is the predominant genotype in sheep in the other parts of the world, this study showed that genotype I is the dominant genotype of in the southern Iran; however, other genotypes were detected. High diversity of genotypes including mix genotypes in lambs is of importance for the public health. These studies depict a new mapping of genotypes pattern which could be very helpful in toxoplasmosis control and prevention.
本研究旨在利用巢式聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，确定伊朗南部绵羊中弓形虫的基因多样性。
2013年4月至6月间，从伊朗法尔斯省南部贾赫罗姆地区的主要屠宰场采集了125只绵羊的膈肌和心脏组织样本。提取DNA后，使用针对SAG2和GRA6基因座的特异性引物进行巢式PCR分析。采用RFLP将菌株分类为弓形虫的三个主要谱系之一。
I型在该地区占主导地位。从两个基因座获得的数据表明，每种基因型的频率分别为：I型72%、III型2.4%、I型和II型混合7.2%、I型和III型混合16.8%、II型和III型混合0.8%、I型、II型和III型混合0.8%。
尽管先前发表的数据表明II型是世界其他地区绵羊中弓形虫的主要基因型，但本研究表明I型是伊朗南部弓形虫的优势基因型；不过，也检测到了其他基因型。包括羔羊混合基因型在内的弓形虫基因型的高度多样性对公共卫生具有重要意义。这些研究描绘了弓形虫基因型模式的新图谱，这对弓形虫病的控制和预防可能非常有帮助。
