Firouzeh Nima, Borj Hamid Foroughi, Ziaali Naser, Kareshk Amir Tavakoli, Ahmadinejad Mohammad, Shafiei Reza
Vector-Borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnourd, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Parasitol. 2023 Apr-Jun;18(2):217-228. doi: 10.18502/ijpa.v18i2.13188.
Toxoplasmosis is a parasitic disease caused by compilation protozoan agent , leading to significant financial and quality-adjusted life-year losses. Overcooked or raw meat consumption has been a considerable transmission route. The present study was conducted to determine the seropositivity rate of in sheep and goats by serological and molecular tests and genotyping of obtained isolates in northeast Iran.
Blood and tissue samples (diaphragm, heart) of 296 animals (including 168 sheep and 128 goats) were collected from the slaughterhouse in Quchan Country from august 2016 to April 2017. Modified agglutination test (MAT) and the PCR method performed to detect parasite DNA on tissues.PCR-RFLP method of gene was used to determine the genotype of . In addition, sequencing analysis was performed to evaluate the type strains.
Serum positive for MAT results were found in 27.4% of sheep and 23.4% of goats. Positive PCR of gene results in diaphragm and heart tissues of sheep and goats was 47.8% and 26.1%, 40% and 23.3%, respectively. PCR of gene results were positive in 10 samples that RFLP technique results using enzyme revealed genotype I. Sequencing and phylogenetic analysis revealed DNA of all samples was closely related to type I.
Concerning the high seropositivity rate of toxoplasmosis, undertaking an appropriate preventive program for reducing the prevalence of infection by raw or undercooked meat consumption of livestock is recommended. Our study supports the notion that these animals' consumption of raw and undercooked meat can be a probable source of human toxoplasmosis.
弓形虫病是一种由复合原生动物病原体引起的寄生虫病，会导致巨大的经济损失和质量调整生命年损失。食用煮得过熟或生的肉类一直是一个重要的传播途径。本研究旨在通过血清学和分子检测确定伊朗东北部绵羊和山羊的弓形虫血清阳性率，并对获得的分离株进行基因分型。
2016年8月至2017年4月，从库詹县的屠宰场采集了296只动物（包括168只绵羊和128只山羊）的血液和组织样本（膈肌、心脏）。采用改良凝集试验（MAT）和PCR方法检测组织中的寄生虫DNA。使用基因的PCR-RFLP方法确定的基因型。此外，进行测序分析以评估型菌株。
MAT检测结果显示，27.4%的绵羊和23.4%的山羊血清呈阳性。绵羊和山羊膈肌和心脏组织中基因PCR阳性率分别为47.8%和26.1%、40%和23.3%。基因PCR结果在10个样本中呈阳性，使用酶的RFLP技术结果显示为I型基因型。测序和系统发育分析表明，所有样本的DNA与I型密切相关。
鉴于弓形虫病的高血清阳性率，建议采取适当的预防措施，以降低因食用生的或未煮熟的家畜肉类而感染的患病率。我们的研究支持这样一种观点，即食用这些动物的生肉和未煮熟的肉可能是人类弓形虫病的一个潜在来源。
