Cui Yupeng, Zhao Yanpeng, Wang Yumei, Liu Zhengjie, Ijaz Babar, Huang Yi, Hua Jinping
Laboratory of Cotton Genetics, Genomics and Breeding, College of Agronomy and Biotechnology/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural UniversityBeijing, China.
Research Institute of Cash Crop, Hubei Academy of Agricultural SciencesWuhan, China.
Front Plant Sci. 2017 May 1;8:624. doi: 10.3389/fpls.2017.00624. eCollection 2017.
Acetyl-CoA carboxylase is an important enzyme, which catalyzes acetyl-CoA's carboxylation to produce malonyl-CoA and to serve as a committed step for fatty acid biosynthesis in plastids. In this study, 24 putative cotton genes were identified based on the lately published genome data in . Among them, 4, 4, 8, and 8 homologs were identified in , and , respectively. These genes were divided into two classes based on a phylogenetic analysis. In each class, these homologs were relatively conserved in gene structure and motifs. The chromosomal distribution pattern revealed that all the genes were distributed equally on corresponding chromosomes or scaffold in the four cotton species. Segmental duplication was a predominant duplication event in both of and The analysis of the expression profile showed that 8 genes expressed in all the tested tissues with changed expression levels, and genes belonging to class II were predominantly expressed in developing ovules. Meanwhile, the expression analysis for the 16 cotton genes from and showed that they were induced or suppressed by cold or salt stress, and their expression patterns varied among different tissues. These findings will help to determine the functional and evolutionary characteristics of the genes in species.
乙酰辅酶A羧化酶是一种重要的酶，它催化乙酰辅酶A的羧化反应生成丙二酰辅酶A，这是质体中脂肪酸生物合成的关键步骤。在本研究中，基于最近发表的基因组数据鉴定出了24个推定的棉花基因。其中，分别在[具体物种1]、[具体物种2]、[具体物种3]和[具体物种4]中鉴定出了4个、4个、8个和8个同源基因。基于系统发育分析，这些基因被分为两类。在每一类中，这些同源基因在基因结构和基序上相对保守。染色体分布模式表明，所有这些基因在四个棉花物种的相应染色体或支架上均匀分布。片段重复是[具体物种1]和[具体物种2]中主要的重复事件。表达谱分析表明，8个基因在所有测试组织中均有表达，且表达水平发生变化，属于II类的基因主要在发育中的胚珠中表达。同时，对来自[具体物种3]和[具体物种4]的16个棉花基因的表达分析表明，它们受到冷胁迫或盐胁迫的诱导或抑制，且其表达模式在不同组织中有所不同。这些发现将有助于确定棉花物种中这些基因的功能和进化特征。
