Hassan Famian Maryam, Montazer Saheb Soheila, Montaseri Azadeh
Department of molecular biology, Ahar Branch, Islamic Azad University, Ahar, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2017 Apr;7(1):123-130. doi: 10.15171/apb.2017.016. Epub 2017 Apr 13.
Mesenchymal stem cells (MSCs) have been introduced for cell therapy strategies in osteoarthritis (OA). Despite of their capacity for differentiation into chondrocyte, there are some evidences about their life-threatening problem after transplantation. So, some researchers shifted on the application of stem cells conditioned medium. The goal of this study is to evaluate whether Wharton's jelly derived stem cell conditioned medium (WJSCs-CM) can enhance the gene expression profile by chondrocytes in monolayer and mass culture systems. Conditioned medium was obtained from WJSCs at fourth passage. Isolated chondrocytes were plated at density of 1×10 for both monolayer and high density culture. Then cells in both groups were divided into control (received medium) and experiment group treated with WJ-CM for 3 and 6 days. Samples were prepared to evaluate gene expression profile of collagen II, aggrecan, cartilage oligomeric matrix protein (COMP) and sox-9 using real-time RT-PCR. After 3 days, Chondrocytes treated with WJSCs-CM expressed significantly higher level of genes compared to the control group in both culture systems. After 6 days, the expression of genes in monolayer cultivated chondrocytes was decreased but that of the mass culture were up-regulated significantly. WJ-SCs-CM can increase the expression of cartilage-specific genes and can be introduced as a promoting factor for cartilage regeneration.
间充质干细胞（MSCs）已被引入用于骨关节炎（OA）的细胞治疗策略。尽管它们具有分化为软骨细胞的能力，但有一些证据表明其移植后存在危及生命的问题。因此，一些研究人员转向了干细胞条件培养基的应用。本研究的目的是评估脐带来源的干细胞条件培养基（WJSCs-CM）是否能增强单层和大规模培养系统中软骨细胞的基因表达谱。条件培养基取自第4代的WJSCs。将分离的软骨细胞以1×10的密度接种于单层和高密度培养中。然后将两组细胞分为对照组（接受培养基）和用WJ-CM处理3天和6天的实验组。使用实时RT-PCR制备样品以评估胶原蛋白II、聚集蛋白聚糖、软骨寡聚基质蛋白（COMP）和sox-9的基因表达谱。3天后，在两种培养系统中，用WJSCs-CM处理的软骨细胞与对照组相比，基因表达水平显著更高。6天后，单层培养的软骨细胞中基因表达下降，但大规模培养的软骨细胞中基因表达显著上调。WJ-SCs-CM可以增加软骨特异性基因的表达，并可作为软骨再生的促进因子引入。
