Han Le, Liu Ben, Chen Xianyan, Chen Haiyan, Deng Wenjia, Yang Changsheng, Ji Bin, Wan Miaojian
Department of Dermatology, The Third Affiliated Hospital, Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, China.
State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, No. 381 Wushan Road, Guangzhou, 510640, China.
Lasers Med Sci. 2018 Apr;33(3):637-645. doi: 10.1007/s10103-018-2455-3. Epub 2018 Feb 21.
Activation of the Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis and hair growth. Recently, low-level laser therapy (LLLT) was evaluated for stimulating hair growth in numerous clinical studies, in which 655-nm red light was found to be most effective and practical for stimulating hair growth. We evaluated whether 655-nm red light + light-emitting diode (LED) could promote human hair growth by activating Wnt/β-catenin signaling. An in vitro culture of human hair follicles (HFs) was irradiated with different intensities of 655-nm red light + LED, 21 h7 (an inhibitor of β-catenin), or both. Immunofluorescence staining was performed to assess the expression of β-catenin, GSK3β, p-GSK3β, and Lef1 in the Wnt/β-catenin signaling. The 655-nm red light + LED not only enhanced hair shaft elongation, but also reduced catagen transition in human hair follicle organ culture, with the greatest effectiveness observed at 5 min (0.839 J/cm). Additionally, 655-nm red light + LED enhanced the expression of β-catenin, p-GSK3β, and Lef1, signaling molecules of the Wnt/β-catenin pathway, in the hair matrix. Activation of Wnt/β-catenin signaling is involved in hair growth-promoting effect of 655-nm red light and LED in vitro and therefore may serve as an alternative therapeutic option for alopecia.
Wnt/β-连环蛋白信号通路的激活在毛囊形态发生和头发生长中起重要作用。最近，在众多临床研究中对低强度激光疗法（LLLT）促进头发生长进行了评估，其中发现655纳米红光在刺激头发生长方面最有效且实用。我们评估了655纳米红光 + 发光二极管（LED）是否能通过激活Wnt/β-连环蛋白信号来促进人类头发生长。用人毛囊（HFs）进行体外培养，分别用不同强度的655纳米红光 + LED、21h7（一种β-连环蛋白抑制剂）或两者进行照射。进行免疫荧光染色以评估Wnt/β-连环蛋白信号中β-连环蛋白、GSK3β、p-GSK3β和Lef1的表达。655纳米红光 + LED不仅增强了毛干伸长，还减少了人毛囊器官培养中的退行期转变，在5分钟（0.839 J/cm）时观察到最大效果。此外，655纳米红光 + LED增强了毛基质中Wnt/β-连环蛋白途径的信号分子β-连环蛋白、p-GSK3β和Lef1的表达。Wnt/β-连环蛋白信号的激活参与了655纳米红光和LED在体外促进头发生长的作用，因此可能成为脱发的一种替代治疗选择。
