Olivieri Lara, Cavina Damiano, Radicchi Giada, Miragliotta Vincenzo, Abramo Francesca
Clinica Veterinaria Modena Sud, Piazza dei Tintori, 41057, Spilamberto, MO, Italy.
Vet Dermatol. 2015 Feb;26(1):35-9, e11. doi: 10.1111/vde.12170. Epub 2014 Sep 16.
Canine noninflammatory alopecia (CNA) is a heterogeneous group of skin diseases with different underlying pathogenesis. The therapeutic approach is challenging, and new options for treatment are desirable.
HYPOTHESIS/OBJECTIVES: To test the clinical efficacy of low-level laser therapy (LLLT) on hair regrowth in CNA.
Seven dogs of different ages, breeds and genders with a clinical and histopathological diagnosis of noninflammatory alopecia.
Each dog was treated twice weekly for a maximum of 2 months with a therapeutic laser producing the following three different wavelengths emerging simultaneously from 21 foci: 13 × 16 mW, 470 nm; 4 × 50 mW, 685 nm; and 4 × 200 mW, 830 nm. The fluence given was 3 J/cm(2) , frequency 5 Hz, amplitude of the irradiated area was 25 cm(2) and application time was 1.34 min. A predetermined alopecic area was left untreated and served as a control area. From one dog, post-treatment biopsies of treated and untreated sites were obtained for histological evaluation of hair density and the percentage of haired and nonhaired follicles.
At the end of the study, coat regrowth was greatly improved in six of seven animals and improved in one of seven. By morphometry, the area occupied by hair follicles was 18% in the treated sample and 11% in the untreated one (11%); haired follicles were (per area) 93% in the treated sample and only 9% in the control sample.
Our clinical and histological data document promising effects of LLLT on hair regrowth in CNA. Further studies investigating the biological mechanism underlying the effect of LLLT on hair follicle cycling are warranted.
犬非炎性脱毛症（CNA）是一组发病机制不同的异质性皮肤疾病。其治疗方法具有挑战性，因此需要新的治疗选择。
假设/目标：测试低强度激光疗法（LLLT）对CNA毛发再生的临床疗效。
7只不同年龄、品种和性别的犬，临床和组织病理学诊断为非炎性脱毛症。
每只犬每周接受两次治疗，最长治疗2个月，使用一种治疗激光，该激光从21个光斑同时发出以下三种不同波长的光：13×16毫瓦，470纳米；4×50毫瓦，685纳米；4×200毫瓦，830纳米。给予的能量密度为3焦/平方厘米，频率为5赫兹，照射面积的振幅为25平方厘米，照射时间为1.34分钟。留出一个预先确定的脱毛区域不进行治疗，作为对照区域。从一只犬身上获取治疗部位和未治疗部位的治疗后活检组织，用于组织学评估毛发密度以及有毛和无毛毛囊的百分比。
在研究结束时，7只动物中有6只的被毛再生情况有显著改善，7只中有1只有所改善。通过形态计量学分析，治疗样本中毛囊所占面积为18%，未治疗样本中为11%；治疗样本中有毛毛囊（每单位面积）为93%，对照样本中仅为9%。
我们的临床和组织学数据证明LLLT对CNA毛发再生有显著效果。有必要进一步研究LLLT对毛囊周期影响的生物学机制。
