Tolszczuk M, Pelletier G
MRC Group in Molecular Endocrinology, Laval University Medical Centre, Sainte-Foy, Que, Canada.
Mol Cell Endocrinol. 1988 Nov;60(1):95-9. doi: 10.1016/0303-7207(88)90123-2.
Catecholamines are known to have an inhibitory effect on oviductal smooth musculature by changing the adrenergic receptors activity. In order to further investigate the role of epinephrine and/or norepinephrine in oviduct, the distribution of beta-adrenergic receptors has been studied in the rat oviduct, using in vitro autoradiography and [125I]cyanopindolol (CYP), as radioligand. The specificity of the labelling and the characterization of receptor subtypes in different cell types was achieved by displacement of radioligand with increasing concentrations of zinterol, a beta-adrenergic agonist with preferential affinity for the beta 2-adrenoreceptor subtype and practolol, a beta-adrenergic antagonist that binds preferentially to beta 1-subtype. Quantitative estimation of ligand binding was achieved by densitometry. It was shown that the vast majority of beta-receptors were of the beta 2-subtype and were found in smooth muscle layers as well as in the epithelium. The latter localization suggests a role for epinephrine and/or norepinephrine on the oviductal epithelium.
已知儿茶酚胺可通过改变肾上腺素能受体活性对输卵管平滑肌组织产生抑制作用。为了进一步研究肾上腺素和/或去甲肾上腺素在输卵管中的作用，利用体外放射自显影技术并使用[125I]氰胍心安（CYP）作为放射性配体，对大鼠输卵管中β-肾上腺素能受体的分布进行了研究。通过用浓度递增的心得乐（一种对β2-肾上腺素能受体亚型具有优先亲和力的β-肾上腺素能激动剂）和心得宁（一种优先与β1-亚型结合的β-肾上腺素能拮抗剂）取代放射性配体，实现了不同细胞类型中标记的特异性和受体亚型的表征。通过光密度测定法对配体结合进行了定量估计。结果表明，绝大多数β受体为β2-亚型，存在于平滑肌层以及上皮中。后者的定位表明肾上腺素和/或去甲肾上腺素对输卵管上皮有作用。
