Stetefeld Jörg, McKenna Sean A, Patel Trushar R
Department of Chemistry, University of Manitoba, 144 Dysart Road, Winnipeg, Manitoba, R3T 2N2, Canada.
Department of Biochemistry and Medical Genetics, University of Manitoba, 745 Bannatyne Avenue - Basic Medical Sciences Building, Winnipeg, Manitoba, R3E 0J9, Canada.
Biophys Rev. 2016 Dec;8(4):409-427. doi: 10.1007/s12551-016-0218-6. Epub 2016 Oct 6.
Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. In this review, we provide evidence of the usefulness of DLS to study the homogeneity of proteins, nucleic acids, and complexes of protein-protein or protein-nucleic acid preparations, as well as to study protein-small molecule interactions. Further, we provide examples of DLS's application both as a complementary method to analytical ultracentrifugation studies and as a screening tool to validate solution scattering models using determined hydrodynamic radii.
动态光散射（DLS），也称为光子相关光谱法（PCS），是研究大分子在溶液中扩散行为的一种非常强大的工具。扩散系数以及由此计算出的流体动力学半径取决于大分子的大小和形状。在本综述中，我们提供了证据，证明DLS在研究蛋白质、核酸以及蛋白质-蛋白质或蛋白质-核酸制剂复合物的均一性，以及研究蛋白质-小分子相互作用方面的有用性。此外，我们还提供了DLS作为分析超速离心研究的补充方法以及作为使用确定的流体动力学半径验证溶液散射模型的筛选工具的应用实例。
