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蛋白质结构研究中酪氨酸侧基的紫外-可见光谱。第2部分：选定的应用。

UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 2: selected applications.

作者信息

Antosiewicz Jan M, Shugar David

机构信息

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, żwirki i Wigury 93, 02-089, Warsaw, Poland.

Institute of Biochemistry & Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.

出版信息

Biophys Rev. 2016 Jun;8(2):163-177. doi: 10.1007/s12551-016-0197-7. Epub 2016 May 4.

DOI:10.1007/s12551-016-0197-7
PMID:28510057
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4884208/
Abstract

In Part 2 we discuss application of several different types of UV-Vis spectroscopy, such as normal, difference, and second-derivative UV absorption spectroscopy, fluorescence spectroscopy, linear and circular dichroism spectroscopy, and Raman spectroscopy, of the side-chain of tyrosine residues in different molecular environments. We review the ways these spectroscopies can be used to probe complex protein structures.

摘要

在第2部分中，我们讨论了几种不同类型的紫外可见光谱在不同分子环境中酪氨酸残基侧链上的应用，如常规紫外吸收光谱、差示紫外吸收光谱、二阶导数紫外吸收光谱、荧光光谱、线性和圆二色光谱以及拉曼光谱。我们回顾了这些光谱用于探测复杂蛋白质结构的方法。

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