蛋白质结构研究中酪氨酸侧基的紫外-可见光谱。第2部分：选定的应用。

UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 2: selected applications.

作者信息

Antosiewicz Jan M, Shugar David

机构信息

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, żwirki i Wigury 93, 02-089, Warsaw, Poland.

Institute of Biochemistry & Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.

出版信息

Biophys Rev. 2016 Jun;8(2):163-177. doi: 10.1007/s12551-016-0197-7. Epub 2016 May 4.

In Part 2 we discuss application of several different types of UV-Vis spectroscopy, such as normal, difference, and second-derivative UV absorption spectroscopy, fluorescence spectroscopy, linear and circular dichroism spectroscopy, and Raman spectroscopy, of the side-chain of tyrosine residues in different molecular environments. We review the ways these spectroscopies can be used to probe complex protein structures.