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蛋白质结构研究中酪氨酸侧链基团的紫外可见光谱。第1部分：酪氨酸发色团的基本原理和性质。

UV-Vis spectroscopy of tyrosine side-groups in studies of protein structure. Part 1: basic principles and properties of tyrosine chromophore.

作者信息

Antosiewicz Jan M, Shugar David

机构信息

Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Żwirki i Wigury 93, 02-089, Warsaw, Poland.

Institute of Biochemistry & Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.

出版信息

Biophys Rev. 2016 Jun;8(2):151-161. doi: 10.1007/s12551-016-0198-6. Epub 2016 May 4.

DOI:10.1007/s12551-016-0198-6
PMID:28510058
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4884207/
Abstract

Spectroscopic properties of tyrosine residues may be employed in structural studies of proteins. Here we discuss several different types of UV-Vis spectroscopy, like normal, difference and second-derivative UV absorption spectroscopy, fluorescence spectroscopy, linear and circular dichroism spectroscopy, and Raman spectroscopy, and corresponding optical properties of the tyrosine chromophore, phenol, which are used to study protein structure.

摘要

酪氨酸残基的光谱特性可用于蛋白质的结构研究。在此，我们讨论几种不同类型的紫外-可见光谱，如常规、差分和二阶导数紫外吸收光谱、荧光光谱、线性和圆二色光谱以及拉曼光谱，以及用于研究蛋白质结构的酪氨酸发色团（苯酚）的相应光学性质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/886e32303df6/12551_2016_198_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/889b34ca6939/12551_2016_198_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/320d832d6506/12551_2016_198_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/e4852709a8c4/12551_2016_198_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/5756958dffa7/12551_2016_198_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/14720217405b/12551_2016_198_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/886e32303df6/12551_2016_198_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/889b34ca6939/12551_2016_198_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/320d832d6506/12551_2016_198_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/e4852709a8c4/12551_2016_198_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/5756958dffa7/12551_2016_198_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/14720217405b/12551_2016_198_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/536d/5418477/886e32303df6/12551_2016_198_Fig7_HTML.jpg

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