Nanobiophotonics & Biomedical Research Laboratory, M.I.S. Electronics Inc., Richmond Hill, ON L4B 1B4, Canada.
Institute for Advanced Non-Destructive and Non-Invasive Diagnostic Technologies (IANDIT), University of Toronto, Toronto, ON M5S 3G8, Canada.
Sensors (Basel). 2024 Aug 20;24(16):5378. doi: 10.3390/s24165378.
This work describes our recent PCB-based plasmonic nanostructured platform patent (US 11,828,747B2) for the detection of biomarkers in breast cancer serum (BCS). A 50 nm thin gold film (TGF) was immersion-coated on PCB (i.e., PCB-TGF) and immobilized covalently with gold nanourchin (GNU) via a 1,6-Hexanedithiol (HDT) linkage to produce a plasmonic activated nanostructured thin film (PANTF) platform. A label-free SERS immunosensor was fabricated by conjugating the platform with monoclonal HER-II antibodies (mAb) in a directional orientation via adipic acid dihydrazide (ADH) to provide higher accessibility to overexpressed HER-II biomarkers (i.e., 2+ (early), 3+ (locally advanced), and positive (meta) in BCS. An enhancement factor (EF) of 0.3 × 10 was achieved for PANTF using Rhodamine (R6G), and the morphology was studied by scanning electron microscopy (SEM) and atomic force microscope (AFM). UV-vis spectroscopy showed the peaks at 222, 231, and 213 nm corresponding to ADH, mAb, and HER-II biomarkers, respectively. The functionalization and conjugation were investigated by Fourier Transform Near Infrared (FT-NIR) where the most dominant overlapped spectra of 2+, 3+, and Pos correspond to OH-combination of carbohydrate, RNH 1st overtone, and aromatic CH 1st overtone of mAb, respectively. SERS data were filtered using the filter from , baseline corrected using the Improved Asymmetric Least Squares () function from the library. The results showed the common peaks at 867, 1312, 2894, 3026, and 3258 cm corresponding to glycine, alanine (C-N-C) assigned to the symmetric C-N-C stretch mode; tryptophan and α helix; C-H antisymmetric and symmetric stretching; NH in amino acids; and N-H stretch primary amide, respectively, with the intensity of Pos > 3 > 2. This trend is justifiable considering the stage of each sample. Principal Component Analysis (PCA) and Linear Discrimination Analysis (LDA) were employed for the statistical analysis of data.
这项工作描述了我们最近基于 PCB 的等离子体纳米结构平台专利（美国专利号 11,828,747B2），用于检测乳腺癌血清中的生物标志物（BCS）。在印刷电路板（即 PCB-TGF）上浸入涂覆 50nm 厚的金膜（TGF），并通过 1,6-己二硫醇（HDT）键合共价固定金纳米棒（GNU），以产生等离子体激活的纳米结构薄膜（PANTF）平台。通过在 ADH 的导向方向上将平台与单克隆 HER-II 抗体（mAb）偶联，制备了无标记 SERS 免疫传感器，以提供更高的 HER-II 生物标志物（即 BCS 中的 2+（早期）、3+（局部进展）和阳性（转移））的可及性。使用 Rhodamine（R6G）对 PANTF 实现了 0.3×10 的增强因子（EF），并通过扫描电子显微镜（SEM）和原子力显微镜（AFM）研究了其形态。紫外可见光谱显示对应于 ADH、mAb 和 HER-II 生物标志物的峰分别在 222、231 和 213nm 处。通过傅里叶变换近红外（FT-NIR）研究了功能化和偶联，其中 2+、3+和 Pos 对应的最主要重叠光谱分别对应于 mAb 的碳水化合物 OH 组合、RNH 第一泛音和芳基 CH 第一泛音。使用 库中的 函数进行 SERS 数据过滤，使用 库中的 函数进行改进的不对称最小二乘（）基线校正。结果显示，对应于甘氨酸、丙氨酸（C-N-C）的峰位于 867、1312、2894、3026 和 3258cm 处，分别对应于对称 C-N-C 伸缩模式；色氨酸和α螺旋；C-H 反对称和对称伸缩；氨基酸中的 NH；和 N-H 伸展一级酰胺，强度顺序为 Pos > 3 > 2。考虑到每个样本的阶段，这种趋势是合理的。主成分分析（PCA）和线性判别分析（LDA）用于数据的统计分析。
