Xu Tianjun, Sun Dianqiao, Sun Yuena, Wang Rixin
Key Laboratory for Marine Living Resources and Molecular Engineering, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China; E-Mails:
Int J Mol Sci. 2011;12(6):4021-6. doi: 10.3390/ijms12064021. Epub 2011 Jun 15.
Expressed sequence tags (ESTs) can be used to identify microsatellite markers. We developed 30 polymorphic microsatellite markers from 5053 ESTs of the Miichthys miiuy. Out of 123 EST derived microsatellites for which PCR primers were designed, 30 loci were polymorphic in 30 individuals from a single natural population with 2-13 alleles per locus. The observed and expected heterozygosities were from 0.1024 to 0.7917 and from 0.2732 to 0.8845, respectively. Nine loci deviated from the Hardy-Weinberg equilibrium, and linkage disequilibrium was significant between 22 pairs of loci. These polymorphic microsatellite loci will be useful for genetic diversity analysis and molecule-assisted breeding for M. miiuy.
表达序列标签（ESTs）可用于识别微卫星标记。我们从鮸鱼的5053个ESTs中开发了30个多态性微卫星标记。在设计了PCR引物的123个EST衍生微卫星中，30个位点在来自单个自然种群的30个个体中具有多态性，每个位点有2 - 13个等位基因。观察到的杂合度和预期杂合度分别为0.1024至0.7917和0.2732至0.8845。9个位点偏离哈迪 - 温伯格平衡，并且在22对位点之间存在显著的连锁不平衡。这些多态性微卫星位点将有助于鮸鱼的遗传多样性分析和分子辅助育种。
