Francis Vanessa I, Stevenson Emma C, Porter Steven L
Biosciences, Geoffrey Pope Building, College of Life and Environmental Sciences, University of Exeter, Exeter EX 4QD, UK.
FEMS Microbiol Lett. 2017 Jun 15;364(11). doi: 10.1093/femsle/fnx104.
Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of infecting a broad range of hosts, in addition to thriving in a broad range of environmental conditions outside of hosts. With this versatility comes the need to tightly regulate its genome to optimise its gene expression and behaviour to the prevailing conditions. Two-component systems (TCSs) comprising sensor kinases and response regulators play a major role in this regulation. This minireview discusses the growing number of TCSs that have been implicated in the virulence of P. aeruginosa, with a special focus on the emerging theme of multikinase networks, which are networks comprising multiple sensor kinases working together, sensing and integrating multiple signals to decide upon the best response. The networks covered in depth regulate processes such as the switch between acute and chronic virulence (GacS network), the Cup fimbriae (Roc network and Rcs/Pvr network), the aminoarabinose modification of lipopolysaccharide (a network involving the PhoQP and PmrBA TCSs), twitching motility and virulence (a network formed from the Chp chemosensory pathway and the FimS/AlgR TCS), and biofilm formation (Wsp chemosensory pathway). In addition, we highlight the important interfaces between these systems and secondary messenger signals such as cAMP and c-di-GMP.
铜绿假单胞菌是一种多能的机会致病菌，除了能在宿主外的多种环境条件下生存外，还能够感染广泛的宿主。鉴于其这种多能性，需要严格调控其基因组，以便根据当前条件优化基因表达和行为。由传感激酶和反应调节因子组成的双组分系统（TCSs）在这种调控中发挥着主要作用。这篇小型综述讨论了越来越多与铜绿假单胞菌毒力相关的TCSs，特别关注多激酶网络这一新兴主题，多激酶网络是由多个共同作用的传感激酶组成的网络，能够感知和整合多种信号以决定最佳反应。深入探讨的网络调控诸如急性和慢性毒力之间的转换（GacS网络）、菌毛（Roc网络和Rcs/Pvr网络）、脂多糖的氨基阿拉伯糖修饰（涉及PhoQP和PmrBA TCSs的网络）、颤动运动和毒力（由Chp化学感应途径和FimS/AlgR TCS形成的网络）以及生物膜形成（Wsp化学感应途径）等过程。此外，我们强调了这些系统与诸如cAMP和c-di-GMP等第二信使信号之间的重要界面。
