Xu Wei-Bin, Liu Yan, Kono Yoshiko, Chang Hsuan, Peng Ching-I, Chung Kuo-Fang
Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences, Guilin, 541006, China.
Herbarium (HAST), Biodiversity Research Center, Academia Sinica, Nangang, Taipei, 115, Taiwan.
Bot Stud. 2013 Dec;54(1):19. doi: 10.1186/1999-3110-54-19. Epub 2013 Aug 27.
Primulina cardaminifolia Yan Liu & W.B. Xu (Gesneriaceae), a distinct new species with imparipinnate leaves, is described and illustrated from a limestone valley in Guangxi Zhuangzu Autonomous Region, China. To assure its generic placement and phylogenetic affinity, phylogenetic analyses were performed using DNA sequences of nuclear ITS and chloroplast trnL-F intron spacer region. Additionally, somatic chromosome number was counted and pollen stainability was tested.
Phylogenetic analyses support its placement in Primulina; however, two phylogenetically distinct ITS sequence types were detected, suggesting a probable hybrid origin. Its pollen stainability is 100% and its chromosome number, 2n = 36, is congruent with all known counts of diploid species of the genus.
All available data support the recognition of the new species Primulina cardaminifolia and suggest that it could have derived from homoploid hybrid speciation. Color plates, line drawings and a distribution map are provided to aid in identification.
描述并展示了产自中国广西壮族自治区一个石灰岩山谷的新种——碎米荠叶报春苣苔（报春花科），其具奇数羽状复叶。为确定其分类地位和系统发育关系，利用核ITS和叶绿体trnL-F内含子间隔区的DNA序列进行了系统发育分析。此外，统计了体细胞染色体数目并测试了花粉可染性。
系统发育分析支持将其置于报春苣苔属；然而，检测到两种系统发育上不同的ITS序列类型，表明可能起源于杂交。其花粉可染性为100%，染色体数目2n = 36，与该属所有已知二倍体物种的计数一致。
所有现有数据支持新种碎米荠叶报春苣苔的认定，并表明它可能起源于同倍体杂交物种形成。提供了彩色图片、线条图和分布图以帮助鉴定。
