从挺水大型植物水生穗莎草中分离和鉴定一种植物毒性物质。
Isolation and identification of a phytotoxic substance from the emergent macrophyte Centrostachys aquatica.
作者信息
Bich Tran Thi Ngoc, Kato-Noguchi Hisashi
机构信息
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, 761-0795, Kagawa, Japan.
Department of Agriculture, The South College of Engineering and Agriculture, Cần Thơ, Vietnam.
出版信息
Bot Stud. 2014 Dec;55(1):59. doi: 10.1186/s40529-014-0059-1. Epub 2014 Aug 12.
BACKGROUND
Centrostachys aquatica is a perennial emergent macrophyte in marshy places and in rivers. The species was recorded in Senegal and Nigeria, but widespread in tropical Africa, and South and East Asia. Aqueous methanol extracts C. aquatica was found to be toxic to several plant species. However, no phytotoxic substance has been reported in this species. Therefore, we investigated phytotoxic activity and searched for phytotoxic substances with allelopathic activity in C. aquatica.
RESULTS
An aqueous methanol extract of C. aquatica inhibited the growth of roots and hypocotyls of cress (Lepidium sativum). The extract was then purified by several chromatographic runs and a phytotoxic substance with allelopathic activity was isolated and identified by spectral analysis as loliolide. Loliolide inhibited cress root and hypocotyl growth at concentrations greater than 0.03 μM. The concentrations required for 50% growth inhibition of cress roots and hypocotyls was 0.18 and 0.15 μM, respectively.
CONCLUSION
These results suggest that loliolide is a phytotoxic substance and may contribute to the allelopathic effect caused by C. aquatica.
背景
水生刺子莞是一种生长在沼泽地和河流中的多年生挺水大型植物。该物种在塞内加尔和尼日利亚有记录，但在热带非洲以及南亚和东亚广泛分布。已发现水生刺子莞的甲醇水提取物对几种植物物种有毒。然而，该物种中尚未有植物毒性物质的报道。因此，我们研究了水生刺子莞的植物毒性活性，并寻找具有化感活性的植物毒性物质。
结果
水生刺子莞的甲醇水提取物抑制了水芹（独行菜）根和下胚轴的生长。然后通过多次色谱分离对提取物进行纯化，并通过光谱分析分离鉴定出一种具有化感活性的植物毒性物质为黑麦草内酯。黑麦草内酯在浓度大于0.03 μM时抑制水芹根和下胚轴的生长。水芹根和下胚轴生长抑制率达50%时所需的浓度分别为0.18 μM和0.15 μM。
结论
这些结果表明黑麦草内酯是一种植物毒性物质，可能导致水生刺子莞产生化感作用。
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