Cranfield Forensic Institute, Cranfield University, Shrivenham, Swindon, SN6 8LA, UK.
Department of Entomology, 243 Natural Science, Michigan State University, East Lansing, MI, USA.
Sci Rep. 2017 May 16;7(1):1933. doi: 10.1038/s41598-017-01667-7.
Cuticular hydrocarbons (CHC) have been successfully used in the field of forensic entomology for identifying and ageing forensically important blowfly species, primarily in the larval stages. However in older scenes where all other entomological evidence is no longer present, Calliphoridae puparial cases can often be all that remains and therefore being able to establish the age could give an indication of the PMI. This paper examined the CHCs present in the lipid wax layer of insects, to determine the age of the cases over a period of nine months. The two forensically important species examined were Calliphora vicina and Lucilia sericata. The hydrocarbons were chemically extracted and analysed using Gas Chromatography - Mass Spectrometry. Statistical analysis was then applied in the form of non-metric multidimensional scaling analysis (NMDS), permutational multivariate analysis of variance (PERMANOVA) and random forest models. This study was successful in determining age differences within the empty cases, which to date, has not been establish by any other technique.
表皮碳氢化合物 (CHC) 已成功应用于法医昆虫学领域，用于鉴定和确定具有法医学重要性的蝇类物种的年龄，主要是在幼虫阶段。然而，在其他所有昆虫学证据都不再存在的旧现场中，Calliphoridae 蛹壳通常是唯一留下的证据，因此能够确定其年龄可以提供 PMI 的指示。本文研究了昆虫脂质蜡层中存在的 CHC，以确定在九个月的时间内蛹壳的年龄。检查的两种具有法医学重要性的物种是 Calliphora vicina 和 Lucilia sericata。使用气相色谱 - 质谱联用技术对碳氢化合物进行化学提取和分析。然后以非度量多维尺度分析 (NMDS)、置换多元方差分析 (PERMANOVA) 和随机森林模型的形式应用统计分析。这项研究成功地确定了空蛹壳内的年龄差异，迄今为止，其他技术尚未确定这一点。
