Wang Xiaohong, Ackermann Maximilian, Neufurth Meik, Wang Shunfeng, Schröder Heinz C, Müller Werner E G
ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, Duesbergweg 6, 55128 Mainz, Germany.
Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University, Johann Joachim Becher Weg 13, D-55099 Mainz, Germany.
Mar Drugs. 2017 May 17;15(5):142. doi: 10.3390/md15050142.
We describe a novel regeneratively-active barrier membrane which consists of a durable electrospun poly(ε-caprolactone) (PCL) net covered with a morphogenetically-active biohybrid material composed of collagen and inorganic polyphosphate (polyP). The patch-like fibrous collagen structures are decorated with small amorphous polyP nanoparticles (50 nm) formed by precipitation of this energy-rich and enzyme-degradable (alkaline phosphatase) polymer in the presence of calcium ions. The fabricated PCL-polyP/collagen hybrid mats are characterized by advantageous biomechanical properties, such as enhanced flexibility and stretchability with almost unaltered tensile strength of the PCL net. The polyP/collagen material promotes the attachment and increases the viability/metabolic activity of human mesenchymal stem cells compared to cells grown on non-coated mats. The gene expression studies revealed that cells, growing onto polyP/collagen coated mats show a significantly (two-fold) higher upregulation of the steady-state-expression of the gene used as an early marker for wound healing than cells cultivated onto non-coated mats. Based on our results we propose that amorphous polyP, stabilized onto a collagen matrix, might be a promising component of functionally-active barrier membranes for guided tissue regeneration in medicine and dentistry.
我们描述了一种新型的具有再生活性的屏障膜，它由耐用的电纺聚（ε-己内酯）（PCL）网组成，上面覆盖着一种由胶原蛋白和无机多聚磷酸盐（polyP）构成的具有形态发生活性的生物杂交材料。片状的纤维状胶原结构点缀着小的无定形多聚磷酸盐纳米颗粒（50纳米），这些纳米颗粒是在钙离子存在的情况下，通过这种富含能量且可被酶降解（碱性磷酸酶）的聚合物沉淀形成的。所制备的PCL-polyP/胶原蛋白混合垫具有有利的生物力学性能，比如柔韧性和拉伸性增强，而PCL网的拉伸强度几乎未变。与在未涂层垫上生长的细胞相比，polyP/胶原蛋白材料促进了人间充质干细胞的附着并提高了其活力/代谢活性。基因表达研究表明，在polyP/胶原蛋白涂层垫上生长的细胞，与在未涂层垫上培养的细胞相比，用作伤口愈合早期标志物的基因的稳态表达上调显著（两倍）。基于我们的研究结果，我们提出，稳定在胶原基质上的无定形多聚磷酸盐可能是医学和牙科引导组织再生功能活性屏障膜的一种有前景的成分。
