Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University, Johann Joachim Becher Weg 13, D-55099, Mainz, Germany.
ERC Advanced Investigator Grant Research Group at the Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz, 55128, Mainz, Germany.
J Biomed Mater Res B Appl Biomater. 2018 Aug;106(6):2109-2121. doi: 10.1002/jbm.b.34016. Epub 2017 Oct 10.
Prostethic mesh material such as nonabsorbable polypropylene used in open and laparoscopic hernia repair are characterized by controllable mechanical properties but may elicit undesirable physiological reactions due to the nonphysiological inert polymer material. We succeeded in developing a biocompatible coating for these meshes, based on a physiological inorganic polymer, polyphosphate (polyP) that is morphogenetically active and used as a metabolic energy source, and a collagen matrix. The polyP/collagen hydrogel material was prepared by a freeze-extraction method, with amorphous Ca-polyP microparticles. Electron microscopy (SEM and REM) studies revealed that the polyP/collagen coats are built up of ≈50 nm-sized microparticles deposited onto the collagen matrix which forms a continuous layer around the polypropylene fibers that also spans the mesh pores. This bioresorbable inorganic/organic hybrid coat was found to be degraded during three days of incubation in medium/serum. The biomechanical properties of the coated meshes are comparable to those of the unmodified polypropylene meshes, with a higher toughness in longitudinal orientation and a more pronounced extensibility in the transverse orientation. The polyP/collagen coating improved cell attachment to the polypropylene meshes and strongly increase the growth of fibroblasts (MC3T3-E1 cells). Furthermore, those mats upregulate the expression of the gene encoding the stromal cell-derived factor-1α (SDF-1), a mesenchymal stem cells attracting chemokine in the fibroblasts. We conclude that coating of inert polymer meshes with a biocompatible, collagen-inducing polyP/collagen inorganic/organic hybrid layer may improve tissue integration of the meshes and the outcome of surgical hernia repair and may redudce the foreign body reaction in contaminated field. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2109-2121, 2018.
用于开放式和腹腔镜疝修补术的不可吸收聚丙烯等假体网片材料的特点是具有可控的机械性能，但由于非生理惰性聚合物材料，可能会引起不良的生理反应。我们成功地为这些网片开发了一种基于生理无机聚合物多磷酸盐（polyP）的生物相容性涂层，polyP 具有形态发生活性，可用作代谢能量源，以及胶原基质。通过冷冻提取方法制备了 polyP/胶原水凝胶材料，其中含有非晶态 Ca-polyP 微米颗粒。电子显微镜（SEM 和 REM）研究表明，polyP/胶原涂层由沉积在胶原基质上的约 50nm 大小的微米颗粒组成，胶原基质在形成连续层的同时环绕着聚丙烯纤维，也跨越了网孔。这种可生物降解的无机/有机杂化涂层在培养基/血清中孵育三天期间被发现会降解。涂层网片的生物力学性能与未改性的聚丙烯网片相当，在纵向方向具有更高的韧性，在横向方向具有更明显的延展性。polyP/胶原涂层可改善细胞对聚丙烯网片的附着，并强烈促进成纤维细胞（MC3T3-E1 细胞）的生长。此外，这些基质上调了基质细胞衍生因子-1α（SDF-1）基因的表达，SDF-1 是一种趋化因子，可吸引成纤维细胞中的间充质干细胞。我们得出结论，用生物相容性的、诱导胶原生成的 polyP/胶原无机/有机杂化层对惰性聚合物网片进行涂层处理可能会改善网片的组织整合以及手术疝修补的效果，并可能减少污染区域的异物反应。© 2017 威利父子公司。J 生物医学材料研究杂志 B：应用生物材料，106B：2109-2121，2018。
