Men S J, Chen C-L, Wei W, Lai T-Y, Song S Z, Wang R K
Department of Bioengineering, University of Washington, Seattle, WA, USA.
Skin Res Technol. 2017 Nov;23(4):607-612. doi: 10.1111/srt.12379. Epub 2017 May 17.
To investigate the repeatability of vessel density measurement at human arm skin in healthy subjects with OCT-based microangiography (OMAG).
Four locations including volar wrist, volar forearm, shoulder, and volar upper arm were scanned using an optimized swept source OCT system, working at center wavelength of 1300 nm and A-line rate of 100 kHz. Three scans were acquired at each location at the same visit. Vascular images of papillary dermis, reticular dermis, and the whole dermis layer were generated with OMAG processing and automatic segmentation algorithms. The vessel density (VD) of each layer was calculated based on vascular images, and the repeatability of the VD at the same physiological location was thereafter assessed.
Fifteen healthy volunteers were included. High repeatability of VD was found for wrist, forearm, shoulder, and upper arm (coefficient of variation (CV)=2.4, 2.7, 2.7, 2.0, and intraclass correlation coefficient (ICC)=0.906, 0.854, 0.943, 0.916 respectively). The VD measurements showed no significant difference between the four locations in any of the three layers, ie papillary layer (P=.1063), reticular layer (P=.3371), and whole dermis layer (P=.3233).
Quantification of VD by using OCT/OMAG is repeatable when imaging skin tissue beds in healthy individuals.
利用基于光学相干断层扫描的微血管造影术（OMAG）研究健康受试者手臂皮肤血管密度测量的可重复性。
使用优化的扫频源光学相干断层扫描系统对包括掌侧腕部、掌侧前臂、肩部和掌侧上臂在内的四个部位进行扫描，该系统的中心波长为1300 nm，A线速率为100 kHz。在同一次就诊时，对每个部位进行三次扫描。通过OMAG处理和自动分割算法生成乳头真皮、网状真皮和整个真皮层的血管图像。根据血管图像计算各层的血管密度（VD），然后评估同一生理部位VD的可重复性。
纳入15名健康志愿者。发现腕部、前臂、肩部和上臂的VD具有高重复性（变异系数（CV）分别为2.4、2.7、2.7、2.0，组内相关系数（ICC）分别为0.906、0.854、0.943、0.916）。在乳头层（P = 0.1063）、网状层（P = 0.3371）和整个真皮层（P = 0.3233）这三层中的任何一层，四个部位之间的VD测量均无显著差异。
在对健康个体的皮肤组织床进行成像时，使用OCT/OMAG对VD进行定量是可重复的。
