Kumari Rina, Khan Mohd Imran, Bhowmick Sourav, Sinha Kislay K, Das Neeladri, Das Prolay
Department of Chemistry, Indian Institute of Technology Patna, Patna 801103, Bihar, India.
Department of Biotechnology, National Institute of Pharmaceutical Education and Research, Hajipur 844102, Bihar, India.
J Photochem Photobiol B. 2017 Jul;172:28-35. doi: 10.1016/j.jphotobiol.2017.05.010. Epub 2017 May 11.
DNA derived well-controlled arrangement of porphyrins has emerged as promising hybrid nanostructures. Exceptional biocompatibility and DNA-directed surface addressability coupled with rich symmetry features of the porphyrin have made these hybrid nanostructures attractive candidates for potential biomedical and biotechnological applications. However, the noteworthy photophysical properties of porphyrin and related molecules when present in DNA based nanostructures are yet to be explored fully and should be a matter of intense research that may unearth a plethora of interesting applications of these nanostructures. Herein, we demonstrate the construction of novel self-assembled DNA-porphyrin hybrid nanonetworks that utilize the porphyrin core for antibacterial applications. Porphyrin derivative with four pendant NH groups was synthesized and conjugated with the 5'-PO of ss-DNA by solution phase phosphoramidation coupling reaction. The conjugation was followed by DNA hybridization mediated self-assembly to form DNA-porphyrin hybrid nanonetwork. The enhanced antimicrobial property of the nanonetwork was envisioned following light irradiation at relevant wavelength. In line with this, comparative antimicrobial activities against gram-negative (Escherichia coli BL-21) and gram-positive bacteria (Staphylococcus aureus) have been studied. Interestingly, DNA-porphyrin nanonetwork afforded highly efficient and coherent photoinduced reactive oxygen species (ROS) generation to display antimicrobial activity against Staphylococcus aureus. The escalated and coherent ROS generation from the nanonetworks was attributed to the ordered placement of the porphyrins that inhibits self-quenching. Our work points out to a good alternative for antibiotic free strategies for preservation of biological materials and other applications.
由DNA实现的卟啉良好控制排列已成为有前景的混合纳米结构。卓越的生物相容性、DNA导向的表面可寻址性以及卟啉丰富的对称特征，使这些混合纳米结构成为潜在生物医学和生物技术应用的有吸引力候选者。然而，当卟啉和相关分子存在于基于DNA的纳米结构中时，其值得注意的光物理性质尚未得到充分探索，应该是深入研究的课题，这可能会发掘出这些纳米结构的大量有趣应用。在此，我们展示了新型自组装DNA-卟啉混合纳米网络的构建，该网络利用卟啉核心用于抗菌应用。合成了具有四个侧链NH基团的卟啉衍生物，并通过溶液相磷酰胺化偶联反应与单链DNA的5'-PO进行共轭。共轭后通过DNA杂交介导的自组装形成DNA-卟啉混合纳米网络。在相关波长光照后，设想纳米网络的抗菌性能会增强。与此一致，研究了对革兰氏阴性菌（大肠杆菌BL-21）和革兰氏阳性菌（金黄色葡萄球菌）的比较抗菌活性。有趣的是，DNA-卟啉纳米网络能高效且连贯地产生光诱导活性氧（ROS），以显示对金黄色葡萄球菌的抗菌活性。纳米网络中ROS产生的增强和连贯归因于卟啉的有序排列，这抑制了自猝灭。我们的工作指出了一种用于保存生物材料和其他应用的无抗生素策略的良好替代方案。
