Tait R C, Rempel H, Rodriguez R L, Kado C I
Gene. 1985;36(1-2):97-104. doi: 10.1016/0378-1119(85)90073-3.
The nucleotide sequence of the probable C terminus of the kanamycin-resistance gene (KmR) and the probable complete sequence of the streptomycin-spectinomycin-resistance gene (SpR) of the IncW plasmid pSa have been determined. The two genes appear to be oriented in the same direction and separated by a spacer region of 53 bp, with transcription proceeding from the KmR gene into the SpR gene. An RNA transcript encompassing the C terminus of the KmR gene, the 53-base spacer, and the N terminus of the SpR gene has the potential to form a stem-loop structure with a free energy value of -68 kcal/mol. The SpR gene of pSa has extensive sequence homology with the aadA gene of the plasmid R538-1. Comparison of the proposed amino acid sequence of the KmR protein of pSa with those of two aminoglycoside phosphotransferases revealed a region of potential homology with those proteins.
已确定IncW质粒pSa的卡那霉素抗性基因（KmR）可能的C末端核苷酸序列以及链霉素-壮观霉素抗性基因（SpR）可能的完整序列。这两个基因似乎同向排列，由一个53 bp的间隔区隔开，转录从KmR基因进入SpR基因。包含KmR基因C末端、53个碱基的间隔区和SpR基因N末端的RNA转录本有可能形成自由能值为-68千卡/摩尔的茎环结构。pSa的SpR基因与质粒R538-1的aadA基因有广泛的序列同源性。将pSa的KmR蛋白的推测氨基酸序列与两种氨基糖苷磷酸转移酶的序列进行比较，发现了与这些蛋白潜在的同源区域。
