通过广谱细菌16S rRNA基因PCR检测培养阴性脑脓肿中的病原菌
Detection of in culture-negative brain abscess by broad-spectrum bacterial 16S rRNA Gene PCR.
作者信息
Chakvetadze Catherine, Purcarea Anastasia, Pitsch Aurelia, Chelly Jonathan, Diamantis Sylvain
机构信息
Infectious Diseases Unit, Centre Hospitalier Marc Jacquet, 2, rue Fréteau de Pény, 77011 Melun, France.
Laboratoire de Bactériologie, Centre Hospitalier Marc Jacquet, 2, rue Fréteau de Pény, 77011 Melun, France.
出版信息
IDCases. 2017 Apr 26;8:94-95. doi: 10.1016/j.idcr.2017.04.013. eCollection 2017.
BACKGROUND
is a strict anaerobic microorganism commensal to the human oropharynx and gastrointestinal tract, which causes a wide spectrum of human diseases and it is an important pathogen in abscesses.
CASE PRESENTATION
We report the case of a previously healthy 64-year-old woman with multiple abscesses due to involving liver, pleura and brain. was not recovered from blood cultures nor from culture of hepatic, pleural and brain drain fluid. The diagnosis was obtained by polymerase chain reaction amplification of bacterial deoxyribonucleic acid in brain abscess drain.
CONCLUSIONS
spp. should be considered in patients with any organ abscess, especially in case of invasive disease with multiple secondary site involving brain.
MOLECULAR
techniques might be of special usefulness in cases that remain negative in culture to obtain the diagnosis and perform adequate treatment.
背景
是一种严格厌氧的微生物，是人类口咽和胃肠道的共生菌，可导致多种人类疾病，是脓肿形成的重要病原体。
病例报告
我们报告了一例既往健康的64岁女性，因累及肝脏、胸膜和脑部而出现多处脓肿。在血培养以及肝、胸膜和脑引流液培养中均未分离出该菌。通过对脑脓肿引流液中的细菌脱氧核糖核酸进行聚合酶链反应扩增得出诊断结果。
结论
对于任何器官脓肿患者，尤其是存在累及脑部等多个继发部位的侵袭性疾病时，应考虑[具体菌种名称]。
分子[技术名称]：在培养结果为阴性的病例中，对于获得诊断和进行适当治疗可能特别有用。
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